DEBLOCK SOLUTION MSDS
MATERIAL SAFETY DATA SHEET
Transgenomic, Inc. Effective Date: April 15, 2003
12325 Emmet Street Document No: MSD-17-1152-XX
Omaha, NE 68164 Revision No: 2
Emergency Phone: 800-424-9300
(CHEMTREC 24hr)
SECTION 1. CHEMICAL IDENTIFICATION
CATALOG #: 17-1152-XX
NAME: DEBLOCK SOLUTION
SUBSTANCE: 5% (w/v) Dichloroacetic Acid in Dichloromethane
SECTION 2. COMPOSITION/INFORMATION ON INGREDIENTS
CAS#: 79-43-6 DICHLOROACETIC ACID
SYNONYMS: DCA; Dichloroethanoic Acid; Acetic Acid, dichloro.
MF: COOHCHCl2
PERCENTAGE: ~5%
EC NUMBER (EINECS): 201-207-0
EC INDEX NUMBER: 607-066-00-5
CAS#: 75-09-2 DICHLOROMETHANE
SYNONYMS: Methylene Chloride, Methane Dichloride, Methylene Dichloride, and
Solmethine
MF: CH2Cl2
PERCENTAGE: ~97%
EC NUMBER (EINECS): 200-838-9
EC INDEX NUMBER: 602-004-00-3
SECTION 3. HAZARDS IDENTIFICATION
LABEL PRECAUTIONARY STATEMENTS: TOXIC, IRRITANT.
ROUTES OF ENTRY: INHALATION, ABSORPTION AND INGESTION
NFPA Ratings (SCALE 0-4): HEALTH=2 FIRE=1 REACTIVITY=0
MAJOR HEALTH HAZARDS: Cancer hazard (humans), respiratory tract irritation, skin
irritation, eye irritation, mucous membrane irritation, blood damage and central
nervous system depression.
POTENTIAL HEALTH EFFECTS:
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-INHALATION: Irritation (possibly severe), nausea, irregular heartbeat, headache,
symptoms of drunkenness, lung congestion, blood disorders, chest pain
reproductive defects, effects on the brain, cancer, difficulty breathing and
digestive disorders.
-SKIN CONTACT: Irritation, absorption may occur, tingling sensation and same effects
reported in other routes of exposure.
-EYE CONTACT: Irritation and same effects reported in other routes of exposure.
-INGESTION: Symptoms of drunkenness, tingling sensation, blood disorders,
convulsions and burns.
-CARCINOGEN STATUS:
-OSHA: Yes.
-NTP: Yes.
-IARC: Yes.
SECTION 4. FIRST-AID MEASURES
SEEK MEDICAL ASSISTANCE FOR ANY OVEREXPOSURE.
INHALATION: If adverse effects occur, remove to uncontaminated area. Give artificial
respiration if not breathing. If breathing is difficult, oxygen should be
administered by qualified personnel. Get immediate medical attention.
SKIN: Wash thoroughly with soap and water. Seek medical attention if burn persists
and/or tissue appears damaged.
EYES: Immediately flush thoroughly with water for at least 15 minutes. Then get
immediate medical attention.
INGESTION: Contact local Poison Control Center or physician immediately.
SECTION 5. FIRE FIGHTING MEASURES
EXTINGUISHING MEDIA: Regular dry chemical or carbon dioxide.
UNUSUAL FIRE AND EXPLOSION HAZARDS: Hazardous polymerization will not
occur. Slight fire hazard.
FLASH POINT: Dichloromethane has no flash point.
For 100% Dichloromethane:
Lower Flammability Limit: 13%
Upper Flammability Limit: 23%
THERMAL DECOMPOSITION PRODUCTS: Halogenated compounds, oxides of
carbon and phosgene.
SECTION 6. ACCIDENTAL RELEASE MEASURES
Do not touch spilled material. Evacuate all unnecessary personnel. Wear a respirator,
chemical safety goggles, rubber boots and rubber gloves. Remove all ignition sources.
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Absorb with sand or other non-combustible absorbent and place in a closed container.
Wash spill site with soapy water.
Notify Local Emergency Planning Committee and State Emergency Response
Commission for releases greater than or equal to RQ (U.S. SARA Section 304).
If release occurs in the U.S. and is reportable under CERCLA Section 103, notify the
National Response Center at (800) 424-8802 (USA) or (202) 426-2675 (USA). The RQ
for 100% Dichloromethane is 1000 lbs.
SECTION 7. HANDLING AND STORAGE
Store and handle in accordance with all current regulations and standards. Keep separated
from incompatible substances. Store in a cool dry place.
SECTION 8. EXPOSURE CONTROLS AND
PERSONAL PROTECTION
EXPOSURE LIMIT for DICHLOROACETIC Acid:
No occupational exposure limits established.
EXPOSURE LIMIT for DICHLOROMETHANE:
25 ppm OSHA TWA
125 ppm OSHA TWA- 15 minutes
12.5 ppm OSHA- Action Level
50 ppm ACGIH TWA
350 mg/m3 (100mL/m3) DFG MAK (peal limitation category-II, 2)
100 ppm (350 mg/m3) UK MEL TWA
300 ppm (1060 mg/m3) UL MEL STEL
MECHANICAL EXHAUST REQUIRED
CHEMICAL SAFETY GOGGLES.
NIOSH/MSHA-APPROVED RESPIRATOR.
SAFETY SHOWER AND EYE BATH.
COMPATIBLE CHEMICAL-RESISTANT GLOVES (RUBBER).
AVOID CONTACT WITH EYES, SKIN AND CLOTHING.
KEEP TIGHTLY CLOSED.
TOXIC
IRRITANT
STORE IN A COOL DRY PLACE.
SECTION 9. PHYSICAL AND CHEMICAL PROPERTIES
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APPEARANCE: Colorless liquid.
BOILING POINT:
For Dichloromethane: 40癈
For Dichloroacetic Acid: 194癈
WATER SOLUBILITY: Slightly Water Soluble.
SPECIFIC GRAVITY:
For Dichloromethane: 1.33g/mL
SECTION 10. STABILITY AND REACTIVITY
REACTIVITY: Stable at normal temperatures and pressure.
CONDITIONS TO AVOID: Avoid heat and inhalation. Minimize contact with
material. Keep out of water supplies and sewers.
INCOMPATIBILITIES: Metals, bases, oxidizing materials and combustible
materials.
HAZARDOUS POLYMERIZATION: WILL NOT OCCUR.
SECTION 11. TOXICOLOGICAL INFORMATION
To the best of our knowledge, the chemical, physical and toxicological properties have
not been thoroughly investigated for this Deblock Solution.
For 100% DICHLOROACETIC ACID (This Solution is only ~ 5%):
CARCINOGEN STATUS: IARC: Human in adequate evidence, animal has limited
evidence, Group 3.
IRRITATION DATA: 2 mg/24 hrs skin-rabbit severe
TOXICITY DATA:
2820 mg/kg oral-rat LD50; 510 礚/kg skin-rabbit LD50
LOCAL EFFECTS: Corrosive: Inhalation, skin eye and ingestion.
ACUTE TOXICITY LEVEL:
MODERATELY TOXIC: Ingestion.
TOXIC: Dermal absorption.
TUMORIGENIC DATA: 100 g/kg oral-rat TDLo/2 years continuous.
MUTAGENIC DATA: Cytogenetic data-mouse lymphocyte 600 mg/L
DNA Damage- mouse-oral 645 mg/kg.
INHALATION: Corrosive substance. May cause severe irritation of
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respiratory tract with coughing, choking, pain and possibly burns of the mucous
membranes. In some cases, pulmonary edema may develop, either immediately
or more often within a period of 5-72 hours. The symptoms may include
tightness in the chest, dyspnea, frothy sputum, cyanosis, and dizziness. Physical
findings may include moist rales, low blood pressure and high pulse pressure.
Severe cases may be fatal.
SKIN CONTACT: Lethal dose reported in rabbits was 510mg/kg. Direct contact may
cause severe irritation, pain and possibly burns.
EYE CONTACT: Direct contact may cause severe irritation, pain and
burns, possible severe. The degree of injury depends on the concentration and
duration of exposure. The full extent of injury may not be immediately apparent.
Repeated of prolonged contact may result in conjunctivitis.
INGESTION: May cause immediate pain and severe burns of the mucous
membranes. There may be discoloration of the tissues. Swallowing and speech
may be difficult at first and then almost impossible. The effects on the esophagus
and gastrointestinal tract may range from irritation to severe corrosion. Edema of
the epiglottis and shock may occur.
For 100% DICHLOROMETHANE (This Solution is ~ 95%):
CARCINOGEN STATUS:
OHSA: Carcinogen.
NTP: Anticipated Human Carcinogen.
IARC: Human Inadequate Evidence, Animal Sufficient Evidence, Group 2B.
ACGIH: A3-Animal Carcinogen.
TRGS 905: K 3.
IRRITATION DATA: 810 mg/24 hrs skin-rabbit severe; 100 mg/24 hrs skin-rabbit
moderate; 162 mg eyes-rabbit moderate; 10 mg eyes-rabbit mild; 500 mg/24 hrs
eyes-rabbit mild.
TOXICITY DATA: 1429 礚/kg oral-man TDLo; 357 mg/kg oral-human LDLo; 500
ppm/1yr intermittent inhalation-human TCLo; 500ppm/8 hrs inhalation-human
TCLo; 1600 mg/kg oral-rat LD50; 52 g/m3 inhalation-rat LC50; 916 mg/kg
intraperitoneal-rat LD50; 350 mg/kg intracheal-rat LDLo.
LOCAL EFFECTS: Irritant via inhalation, skin and eye contact.
TOXICITY LEVEL:
MODERATELY TOXIC: inhalation and ingestion.
TARGET ORGANS: Blood and Central Nervous System.
TUMORIGENIC DATA: 3,500 ppm inhalation-rat TCLo/6 hrs over 2 years intermittent;
2,000 ppm inhalation-mouse TCLo/5 hrs over 2 years continuous.
MUTAGENIC DATA: DNA inhibition-human fibroblast 5,000 ppm 1 hr continuous;
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morphological transformation-rat embryo 160 祄ol/L; DNA damage-rat oral
1,275 mg/kg; morphological transformation-hamster embryo 1,300 礚/plate;
DNA damage-hamster ovary 3,000 ppm; mutation in mammalian somatic cells-
hamster ovary 3,000 ppm.
REPRODUCTIVE EFFECTS DATA: 4,500 ppm inhalation-rat TCLo/24 hrs 1-17 days
pregnant female continuous; 1250 ppm inhalation-rat TCLo/7 hrs 6-16 days
pregnant female continuous.
INHALATION: Human exposure to 100 ppm has resulted in upper respiratory tract
irritation; concentrations as low as 200 ppm have produced temporary
neurobehavioural effects; 500-1000 ppm for 1-2 hrs has caused lightheadedness
and elevated carboxyhemoglobin level; 2,300 ppm for 30 minutes has caused
nausea and narcosis; 5000 ppm has caused headache, fatigue, neurasthenic
disorders and digestive disturbances. Other symptoms may include dizziness,
tingling, numbness of extremities, a sensation of fullness in the head,
drunkenness, stupor, dullness, and mental confusion. Massive exposure may
cause pharyngeal erosion, pulmonary edema, staggering, hemolysis with gross
hematuria, rapid unconsciousness and death. Exposure to high levels may also
cause cardiac arrhythmias.
SKIN CONTACT: Effects may range from mild irritation to severe pain, paresthesias,
and possibly burns. Skin absorption may occur causing effects detailed in
inhalation. Prolonged or repeated contact may cause dry, scaly and fissured
dermatitis due to defatting action of liquid on skin.
EYE CONTACT: Vapor concentrations above 2,000 ppm may cause irritation. Direct
contact may cause pain and extreme irritation, but is not likely to cause serious
injury. 10mg applied to rabbit eyes produced keratitis, iritis, increased corneal
thickness, and inflammation of the conjunctiva and eyelids with some effects
lasting up to two weeks.
INGESTION: May cause rapid, then slowed respiration, glottal and pharyngeal edema,
intravascular hemolysis with gross hematuria, gastrointestinal ulceration and
hemorrhage, and carboxyhemoglobinemia. In addition to possible corresponding
pneumonia, symptoms of central nervous system depression may occur followed
by convulsions and paresthesia of the extremities. Large doses may cause liver
and kidney damage. The estimated lethal dose for an adult is 25 g.
SECTION 12. ECOLOGICAL INFORMATION
ECOTOXICITY DATA (FOR 100% Dichloromethane):
FISH TOXICITY: 193 mL/L 96 hrs LC50 (Mortality) Fathead Minnow
(Pimephales promelas).
OTHER TOXICITY: 177.8 mL/L 8 hours EC50 (Teratogenesis) Bullfrog (Rana
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catesbeiana).
ENVIRONMENTAL SUMMARY: Harmful to aquatic life. Relatively non-persistent in
the environment. Not expected to leach through the soil or the sediment.
Accumulates very little in the bodies of living organisms. Highly volatile from
water.
SECTION 13. DISPOSAL CONSIDERATIONS
Dispose in accordance with all applicable regulations.
SECTION 14. TRANSPORTATION INFORMATION
U.S. DOT 49 CFR 172.101:
HAZARD CLASS OR DIVISION: 6.1
PROPER SHIPPING NAME: Toxic liquid, organic, n.o.s. (Contains
Dichloromethane)
UN ID Number: 2810
PACKING GROUP: III
LABELING REQUIREMENTS: TOXIC
AIR (I.C.A.O.): Same as Above.
INTERNATIONAL (I.M.O.): Same as Above.
SECTION 15. REGULATORY INFORMATION
FOR 100% Dichloromethane:
TSCA INVENTORY STATUS: Yes.
TSCA 12 (b) EXPORT NOTIFICATION: Not listed.
CERCLA SECTION 102a/103 (40CFR302.4): Yes, 1000 lbs RQ.
SARA SECTION 302 (40CFR355.30): Not Regulated.
SARA SECTION 304 (40CFR355.40): Not Regulated.
SARA HAZARD CATEGORIES, SARA SECTIONS 311/312 (40CFR370.21):
ACUTE: Yes.
CHRONIC: Yes.
FIRE: No.
REACTIVE: No.
SUDDEN RELEASE: No.
SARA SECTION 313 (40CFR372.65): Yes.
OSHA PROCESS SAFETY (29CFR1910.119): Not Regulated.
STATE REGULATIONS:
CALIFORNIA PROPOSITION 65: Yes. Known to the state of California
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to cause the following: Cancer (April 01, 1988).
CANADIAN REULATIONS:
WHMIS CLASSIFICATION: Not Determined.
EUROPEAN REGULATIONS:
EC CLASSIFICATIONS: Assigned:
Carcinogen Category 3.
DANGER/HAZARD SYMBOL: Xn Harmful
EC RISK AND SAFETY PHRASES:
R 40 Limited evidence of carcinogenic effect.
S2 Keep out of reach of children.
S 23 Do not breath gas, fumes, vapor or spray.
S 24/25 Avoid contact with skin and eyes.
S 36/37 Wear suitable protective clothing and gloves.
GERMAN REGULALTIONS:
Water Hazard Class (WGK): 2
SECTION 16. OTHER INFORMATION
The above information is believed to be correct and shall be used only as a guide. The
information may be inclusive. Transgenomic, Inc. shall not be held liable for any
damage resulting from handling or from contact with the above material.
2032 CONCOURSE DRIVE 12325 EMMET STREET THE QUADRANGLE,CREWE HALL WESTON RD. 8-14-21 NISHI-SHINJUKU SHINJUKU-KU
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