Giemsa Stain Solution
Material Safety Data Sheet
Section 1. Product and Company Identification
Product Name Giemsa Stain Solution Product 620
Code
Manufacturer EMD Chemicals Inc. Effective 3/4/2003
P.O. Box 70 Date
480 Democrat Road
Gibbstown, NJ 08027
Prior to January 1, 2003 EMD Chemicals Inc. was
EM Industries, Inc. or EM Science, Division of
EM Industries, Inc.
For More Information Call In Case of Emergency Call
856-423-6300 Technical Service 800-424-9300 CHEMTREC
Monday-Friday: 8:00 AM - 5:00 PM (USA)
613-996-6666 CANUTEC
(Canada)
24 Hours/Day: 7 Days/Week
Synonym None.
Material Uses Laboratory Reagent
Chemical Dye Solution
Family
Section 2. Composition and Information on Ingredients
Component CAS # % by
Weight
Giemsa Stain 51811-82-6
GLYCERIN 56-81-5 35-44
METHANOL 67-56-1 50-55
Section 3. Hazards Identification
Physical State and Liquid.
Appearance
Emergency DANGER !POISON !
Overview FLAMMABLE LIQUID AND VAPOR.
VAPOR MAY CAUSE FLASH FIRE.
MAY BE FATAL IF SWALLOWED.
HARMFUL IF INHALED.
CANNOT BE MADE NON-POISONOUS
CONTAINS MATERIAL WHICH CAUSES DAMAGE TO THE
FOLLOWING ORGANS: KIDNEYS, GASTROINTESTINAL TRACT,
RESPIRATORY TRACT, SKIN, CENTRAL NERVOUS SYSTEM,
EYE, LENS OR CORNEA.
Routes of Entry Inhalation. Ingestion.
Potential Acute Health
Effects
Eyes No known effect on eye contact, rinse with water for a few minutes.
Skin No known effect on skin contact; rinse with water for a few minutes.
Inhalation Hazardous in case of inhalation.
Ingestion Extremely hazardous in case of ingestion. May be fatal if swallowed.
Potential Chronic Health Effects
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Giemsa Stain Solution
Carcinogenic This material is not known to cause cancer in animals or humans.
Effects
Additional information See Toxicological Information (section 11)
Medical Conditions Repeated exposure to a highly toxic material may produce general
Aggravated by deterioration of health by an accumulation in one or many human organs.
Overexposure:
Section 4. First Aid Measures
Eye Contact Check for and remove any contact lenses. In case of contact, immediately
flush eyes with plenty of water for at least 15 minutes. Cold water may be
used. Get medical attention.
Skin Contact In case of contact, immediately flush skin with plenty of water. Remove
contaminated clothing and shoes. Cold water may be used.Wash clothing
before reuse. Thoroughly clean shoes before reuse. Get medical attention.
Inhalation If inhaled, remove to fresh air. If not breathing, give artificial respiration.
If breathing is difficult, give oxygen. Get medical attention immediately.
Ingestion If swallowed, do not induce vomiting unless directed to do so by medical
personnel. Never give anything by mouth to an unconscious person.
Loosen tight clothing such as a collar, tie, belt or waistband. Get medical
attention immediately.
Section 5. Fire Fighting Measures
Flammability of the Product will burn.
Product
Auto-ignition The lowest known value is 369.9癈 (697.8癋) (GLYCERIN).
Temperature
Flash Points Closed cup: 11癈 (51.8癋).
Flammable Limits The greatest known range is LOWER: 6% UPPER: 36.5%
(METHANOL)
Products of These products are carbon oxides (CO, CO2).
Combustion
Fire Hazards in Highly flammable in presence of open flames, sparks and static
Presence of Various discharge, of shocks, of heat, of oxidizing materials.
Substances
Explosion Hazards Risks of explosion of the product in presence of static discharge:
in Presence of Highly flammable in presence of open flames, sparks and static discharge.
Various Substances Highly explosive in presence of open flames, sparks and static discharge.
Risks of explosion of the product in presence of mechanical impact:
Highly flammable in presence of shocks.
Highly explosive in presence of shocks.
Fire Fighting Media SMALL FIRE: Use DRY chemical powder.
and Instructions LARGE FIRE: Use alcohol foam, water spray or fog. Cool containing
vessels with water jet in order to prevent pressure build-up, autoignition
or explosion.
Protective Clothing Be sure to use an approved/certified respirator or equivalent.
(Fire)
Special Remarks Vapor may travel considerable distance to source of ignition and flash
on Fire Hazards back.
Special Remarks on Development of hazardous combustion gases or vapors possible in the
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Giemsa Stain Solution
Explosion Hazards event of fire.
Section 6. Accidental Release Measures
Small Spill and Dilute with water and mop up, or absorb with an inert dry material and
Leak place in an appropriate waste disposal container.
Large Spill and Keep away from heat. Keep away from sources of ignition. Stop leak if
Leak without risk. Cover with DRY earth, DRY sand or other non-combustible
material followed with plastic sheet to minimize spreading or contact with
rain. Do not get water inside container. Do not touch spilled material. Use
water spray to reduce vapors. Prevent entry into sewers, basements or
confined areas; dike if needed. Call for assistance on disposal. Be careful
that the product is not present at a concentration level above TLV. Check
TLV on the MSDS and with local authorities.
Spill Kit The following EM SCIENCE SpillSolv (TM) absorbent is recommended
Information for this product:
SX1330 Solvent Treatment Kit
Section 7. Handling and Storage
Handling Keep away from heat, sparks and flame. Keep container closed. Use only
with adequate ventilation. To avoid fire or explosion, dissipate static
electricity during transfer by grounding and bonding containers and
equipment before transferring material. Use explosion-proof electrical
(ventilating, lighting and material handling) equipment.
Storage Store in a segregated and approved area. Keep container in a cool,
well-ventilated area. Keep container tightly closed and sealed until ready
for use. Avoid all possible sources of ignition (spark or flame).
Section 8. Exposure Controls/Personal Protection
Engineering Provide exhaust ventilation or other engineering controls to keep the
Controls airborne concentrations of vapors below their respective threshold limit
value. Ensure that eyewash stations and safety showers are proximal to
the work-station location.
Personal Protection
Eyes Safety glasses.
Body Lab coat.
Respiratory Vapor respirator. Be sure to use an approved/certified respirator or
equivalent. Wear appropriate respirator when ventilation is inadequate.
Hands Gloves.
Feet Not applicable.
Protective Clothing
(Pictograms)
Personal Protection Splash goggles. Full suit. Vapor respirator. Boots. Gloves. A
in Case of a Large self-contained breathing apparatus should be used to avoid inhalation of
Spill the product. Suggested protective clothing might not be sufficient; consult
a specialist BEFORE handling this product.
Product Name Exposure Limits
Giemsa Stain Not available.
GLYCERIN Belgium Minister of Labour (Belgium, 1998).
VL: 10 mg/m3
Tyterveyslaitos (Finland, 1998).
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Giemsa Stain Solution
TWA: 20 mg/m3
INRS (France, 1996).
VME: 10 mg/m3
National Authority for Occupational Safety/Health
(Ireland, 1999).
OEL: 10 mg/m3
Arbeidsinspectie (Netherlands, 1999).
TGG 8 uur: 10 mg/m3
EH40-OES (United Kingdom (UK), 1997).
TWA: 10 mg/m3
ACGIH (United States, 1994).
TWA: 10 mg/m3 Form: Mist
OSHA Final Rule (United States, 1989).
TWA: 5 mg/m3 Form: Respirable fraction
TWA: 10 mg/m3 Form: Total dust
METHANOL ACGIH (United States, 1994). Skin
TWA: 262 mg/m3
STEL: 328 mg/m3
OSHA (United States, 1989). Skin
TWA: 260 mg/m3
STEL: 325 mg/m3
ACGIH (United States, 1994). Skin
STEL: 328 mg/m3
STEL: 250 ppm
TWA: 262 mg/m3
TWA: 200 ppm
NIOSH REL (United States, 1994). Skin
STEL: 325 mg/m3
STEL: 250 ppm
TWA: 260 mg/m3 Period: 10 hour(s).
TWA: 200 ppm Period: 10 hour(s).
OSHA Final Rule (United States, 1989). Skin
STEL: 325 mg/m3
STEL: 250 ppm
TWA: 260 mg/m3
TWA: 200 ppm
Section 9. Physical and Chemical Properties
Odor Alcohol like. (Slight.)
Color Purple.
Physical State and Liquid.
Appearance
Molecular Weight Not applicable.
Molecular Formula Not applicable.
pH Not available.
Boiling/Condensation The lowest known value is 64.55癈 (148.2癋) (METHANOL).
Point Weighted average: 161.37癈 (322.5癋)
Melting/Freezing May start to solidify at 19.9癈 (67.8癋) based on data for: GLYCERIN.
Point Weighted average: -47.22癈 (-53癋)
Specific Gravity Weighted average: 0.94 (Water = 1)
Vapor Pressure
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Giemsa Stain Solution
The highest known value is 12.9 kPa (97 mmHg) (@ 20癈)
(METHANOL).
Vapor Density The highest known value is 3.1 (Air = 1) (GLYCERIN). Weighted
average: 1.96 (Air = 1)
Volatility 99.9% (v/v). (METHANOL.)
Odor Threshold The highest known value is 100 ppm (METHANOL)
Evaporation Rate 5.91 (METHANOL) compared to (n-BUTYL ACETATE=1)
VOC 100 (%)
LogKow Not available.
Solubility Soluble in water.
Section 10. Stability and Reactivity
Stability and The product is stable.
Reactivity
Conditions of Not available.
Instability
Incompatibility Highly reactive with oxidizing agents, reducing agents, combustible
with Various materials, alkalis.
Substances Reactive with metals, acids.
Rem/Incompatibility Avoid contact with strong oxiders, excessive heat, sparks or open flame.
Hazardous COx , Formaldehyde.
Decomposition
Products
Hazardous Will not occur.
Polymerization
Section 11. Toxicological Information
RTECS Number:
Giemsa Stain Not available.
Glycerol MA8050000
Methanol PC1400000
Toxicity Acute oral toxicity (LD50): 4090 mg/kg [Mouse]. (GLYCERIN).
Acute dermal toxicity (LD50): 15800 mg/kg [Rabbit]. (METHANOL).
Acute toxicity of the vapor (LC50): 64000 ppm 4 hour(s) [Rat].
(METHANOL).
Chronic Effects on Not available.
Humans
Acute Effects on Non-permeator by skin. Hazardous in case of inhalation. Extremely
Humans hazardous in case of ingestion. May be fatal if swallowed.
Synergetic Products Not available.
(Toxicologically)
Irritancy Draize Test: Not available.
Sensitization Not available.
Carcinogenic This material is not known to cause cancer in animals or humans.
Effects
Toxicity to Not available.
Reproductive
System
Teratogenic Effects Not available.
Mutagenic Effects Not available.
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Giemsa Stain Solution
Section 12. Ecological Information
Ecotoxicity Not available.
BOD5 and COD Not available.
Toxicity of the The products of degradation are less toxic than the product itself.
Products of
Biodegradation
Section 13. Disposal Considerations
EPA Waste D001 U154
Number
Treatment Incineration, fuels blending or recycle. Contact your local permitted waste
disposal site (TSD) for permissible treatment sites. ALWAYS CONTACT
PERMITTED WASTE DISPOSER (TSD) TO ASSURE COMPLIANCE
WITH ALL CURRENT LOCAL, STATE AND FEDERAL
REGULATIONS.
Section 14. Transport Information
DOT Classification Proper Shipping Name: METHANOL
SOLUTION
Hazard Class: 3
UN number: UN1230
Packing Group: II
RQ: Not applicable.
TDG Classification Not available.
IMO/IMDG Not available.
Classification
ICAO/IATA Not available.
Classification
Section 15. Regulatory Information
U.S. Federal TSCA 8(b) inventory: GLYCERIN; METHANOL
Regulations
SARA 302/304/311/312 extremely hazardous substances: No products
were found.
SARA 302/304 emergency planning and notification: No products were
found.
SARA 302/304/311/312 hazardous chemicals: GLYCERIN; METHANOL
SARA 311/312 MSDS distribution - chemical inventory - hazard
identification: GLYCERIN: Immediate (Acute) Health Hazard, Delayed
(Chronic) Health Hazard; METHANOL: Fire Hazard, Immediate (Acute)
Health Hazard, Delayed (Chronic) Health Hazard
SARA 313 toxic chemical notification and release reporting: METHANOL
52.5%
Clean Water Act (CWA) 307: No products were found.
Clean Water Act (CWA) 311: No products were found.
Clean air act (CAA) 112 accidental release prevention: No products were
found.
Clean air act (CAA) 112 regulated flammable substances: No products
were found.
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Giemsa Stain Solution
Clean air act (CAA) 112 regulated toxic substances: No products were
found.
WHMIS (Canada) CLASS B-3: Combustible liquid with a flash point between 37.8癈
(100癋) and 93.3癈 (200癋).
Class D-1A: Material causing immediate and serious toxic effects (VERY
TOXIC).
CEPA DSL: GLYCERIN; METHANOL
This product has been classiifed in accordance with the hazard criteria of
the Controlled Product Regulations and the MSDS contains all required
information.
International
Regulations
EINECS Giemsa Stain Not available.
GLYCERIN 200-289-5
METHANOL 200-659-6
DSCL (EEC) R11- Highly flammable.
R23/24/25- Toxic by inhalation, in contact with skin and if swallowed.
R39/23/24/25- Toxic: danger of very serious irreversible effects through
inhalation, in contact with skin and if swallowed.
International Australia (NICNAS): GLYCERIN; METHANOL
Lists
Japan (MITI): GLYCERIN; METHANOL
Korea (TCCL): GLYCERIN; METHANOL
Philippines (RA6969): GLYCERIN; METHANOL
China: No products were found.
State Regulations Pennsylvania RTK: GLYCERIN: (generic environmental hazard);
METHANOL: (environmental hazard, generic environmental hazard)
Massachusetts RTK: GLYCERIN; METHANOL
New Jersey: Giemsa Stain Solution
California prop. 65: No products were found.
Section 16. Other Information
National Fire 3 0
Protection
1
Association Health
(U.S.A.)
Fire Hazard
Reactivity
Specific Hazard
Changed Since Last
+
Revision
Notice to Reader
The statements contained herein are based upon technical data that EMD Chemicals Inc.
believes to be reliable, are offered for information purposes only and as a guide to the
appropriate precautionary and emergency handling of the material by a properly trained
person having the necessary technical skills. Users should consider these data only as a
supplement to other information gathered by them and must make independent
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Giemsa Stain Solution
determinations of suitability and completeness of information from all sources to assure
proper use, storage and disposal of these materials and the safety and health of employees
and customers and the protection of the environment. EMD CHEMICALS INC. MAKES
NO REPRESENTATION OR WARRANTY OF ANY KIND, EXPRESS OR IMPLIED,
INCLUDING MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE, WITH
RESPECT TO THE INFORMATION HEREIN OR THE PRODUCT TO WHICH THE
INFORMATION REFERS.
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