OXIS International, Inc.
MATERIAL SAFETY DATA SHEET (page 1 of 7)
SECTION I - MANUFACTURER AND PRODUCT IDENTITY
Manufacturer's Name: OXIS International, Inc.
6040 N. Cutter Circle, Suite 317
Portland, OR 97217-3935
Telephone Number: In Oregon (503) 283-3911
Toll Free (800) 547-3686
Facsimile (503) 283-4058
8-OHdG ELISA Assay Kit
Common Name/Trade Name:
Catalog Number(s): 21026
Date: September 2003
SECTION II - HAZARDOUS COMPONENTS IDENTITY
Hazards identified with this product are associated with the following Assay Kit component(s):
Component: Hazardous Content:
Component: 8-OhdG Microtiter Plate
8-OhdG Microtiter Plate
Name: 8-hydroxy-2'-deoxy-guanosine
CAS#: 88847-89-6
MF: C10H13N5O5
Component: Chromogen
Chromogen
Name: 3,3',5,5'-Tetramethylbenzidine (TMB)
CAS#: 54827-17-7
MF: C16H20N2
Name: Sodium Azide (0.1%)
CAS#: 26628-22-8
MF: NaN3
Component: Chromogen Dilution Buffer
Chromogen Dilution Buffer
Name: Hydrogen Peroxide, (0.1%)
CAS#: 7722-84-1
MF: H2O2
Name: Citrate-Phospate Buffered Saline
CAS#: N/A
MF: N/A
Name: Sodium Azide (0.1%)
CAS#: 26628-22-8
MF: NaN3
Component: Primary Antibody
Primary Antibody
Name: Anti 8-OHdG mouse monoclonal
antibody (Type:IgG1)
CAS#: N/A
MF: Unknown
Name: Polyoxyethylene (20) Sorbitan
Monolaurate
CAS#: 9005-64-5
MF: C58H114O26
Msds21026 8-OHdG ELISA Assay Kit.doc
OXIS International, Inc.
MATERIAL SAFETY DATA SHEET (page 2 of 7)
Component: Primary Antibody Dilution Buffer
Primary Antibody Dilution Buffer
Name: Phosphate Buffered Saline
CAS#: None
MF: N/A
Name: Sodium Azide (0.1%)
CAS#: 26628-22-8
MF: NaN3
Component: Secondary Antibody
Secondary Antibody
Name: HRP-conjugated antibody
CAS#: N/A
MF: Unknown
Component: Secondary Antibody Dilution Buffer
Secondary Antibody Dilution Buffer
Name: Phosphate Buffered Saline
CAS#: None
MF: N/A
Name: Sodium Azide
CAS#: 26628-22-8
MF: NaN3
Component: Standard 1-6
Standard 1-6
Name: 8-hydroxy-2'-deoxy-guanosine
CAS#: 88847-89-6
MF: C10H13N5O5
Name: Sodium Azide (0.1%)
CAS#: 26628-22-8
MF: NaN3
Component: Stop Solution
Stop Solution
Name: Phosphoric Acid (1 M)
CAS#: 7664-38-2
MF: H3O4P
Name: Sodium Azide (0.1%)
CAS#: 26628-22-8
MF: NaN3
Component: Wash Buffer
Wash Buffer
Name: Phosphate Buffered Saline
CAS#: None
MF: N/A
Name: Polyoxyethylene (20) Sorbitan
Monolaurate
CAS#: 9005-64-5
MF: C58H114O26
Name: Sodium Azide (0.1%)
CAS#: 26628-22-8
MF: NaN3
Msds21026 8-OHdG ELISA Assay Kit.doc
OXIS International, Inc.
MATERIAL SAFETY DATA SHEET (page 3 of 7)
SECTION III - PHYSICAL AND CHEMICAL CHARACTERISTICS
(All values are approximate)
3,3',5,5'-Tetramethylbenzidine (TMB):
Melting point: 168 C to 169 C Flash point: N/A
Specific gravity: N/A Flammable limits: N/A
Vapor pressure: N/A Extinguisher media: CO2, Dry Chemical, Foam,H2O
Vapor density: N/A Auto-ignition temperature: N/A
Water solubility: Soluble
Reactivity in water N/A
Appearance: White to yellow powder
Odor: N/A
8-Hydroxy-2'-deoxyguanosine:
Boiling point: N/A Flash point: N/A
Specific gravity: N/A Flammable limits: N/A
Vapor pressure: N/A Extinguisher media: CO2, Dry chemical, Foam, H2O
Vapor density: N/A Auto-ignition temperature: N/A
<50 礸/mL @ 25 C
Water solubility:
Reactivity in water N/A
Appearance: Crysytalline solid
Odor: N/A
Citrate-Phosphate Buffered Saline:
Boiling point: N/A Flash point: N/A
Specific gravity: N/A Flammable limits: N/A
Vapor pressure: N/A Extinguisher media: CO2, Dry chemical, Foam, H2O
Vapor density: N/A Auto-ignition temperature: N/A
Water solubility: Soluble
Reactivity in water N/A
Appearance: Liquid
Odor: Odorless
Hydrogen Peroxide 30%:
Boiling point: 106癈 Flash point: N/A
Specific gravity: 1.11 Flammable limits: N/A
Vapor pressure: 24.8 mmHg Extinguisher media: Water
Vapor density: 1.0 Auto-ignition temperature: N/A
Water solubility: Miscible
Reactivity in water N/A
Appearance: Colorless liquid
Odor: slight odor
Phosphate Buffered Saline:
Boiling point: N/A Flash point: N/A
Specific gravity: N/A Flammable limits: N/A
Vapor pressure: N/A Extinguisher media: CO2, Dry chemical, Foam, H2O
Vapor density: N/A Auto-ignition temperature: N/A
Water solubility: Soluble
Reactivity in water N/A
Appearance: Liquid
Odor: Odorless
Phosphoric Acid:
Boiling point: 261癈 Flash point: N/A
Specific gravity: 1.69 Flammable limits: N/A
Vapor pressure: 0.0285 mmHg Extinguisher media: CO2, Dry chemical, Foam, H2O
Vapor density: N/A Auto-ignition temperature: N/A
Water solubility: Miscible
Reactivity in water N/A
Appearance: Colorless liquid
Odor: Odorless
Msds21026 8-OHdG ELISA Assay Kit.doc
OXIS International, Inc.
MATERIAL SAFETY DATA SHEET (page 4 of 7)
Polyoxyethylene (20) Sorbitan Monolaurate:
Boiling point: N/A Flash point: 321癈
Specific gravity: 1.080 Flammable limits: N/A
Vapor pressure: N/A Extinguisher media: CO2, Dry chemical, Foam, H2O
Vapor density: N/A Auto-ignition temperature: N/A
Water solubility: Soluble
Reactivity in water N/A
Appearance: Yellowish brown clear/slightly muddy liquid
Odor: N/A
Sodium Azide:
Boiling point: N/A Flash point: N/A
Specific gravity: 1.846 Flammable limits: N/A
Vapor pressure: N/A Extinguisher media: CO2, Dry chemical, Foam, H2O
Vapor density: N/A Auto-ignition temperature: N/A
Water solubility: Soluble
Reactivity in water N/A
Appearance: White/nearly white crystalline powder
Odor: N/A
SECTION IV - REACTIVITY DATA AND STORAGE CONDITIONS
3,3',5,5'-Tetramethylbenzidine (TMB):
Stability: Stable.
Incompatibilities: Protect from light. Protect from moisture. Avoid contact with
metals, strong acids, strong oxidizing agents.
Storage conditions: Store in Nitrogen. Hygroscopic. Store tightly capped in the
dark at room temperature, preferably cool.
Hazardous decomposition products: Carbon Monoxide,Carbon Dioxide, Nitrogen Oxides.
Hazardous polymerization: Will not occur.
8-Hydroxy-2'-deoxyguanosine:
Stability: Stable.
Incompatibilities: Extremely acidic conditions may cause this compound to
deglycosilate.
Storage conditions: Store tightly capped.
Hazardous decomposition products: N/A
Hazardous polymerization: Will not occur.
Citrate-Phosphate Buffered Saline:
Stability: Stable.
Incompatibilities: Oxidizers, acids
Storage conditions: Store tightly capped in the dark at room temperature.
Hazardous decomposition products: Phosphor Oxides and Sodium compounds.
Hazardous polymerization: Will not occur.
Hydrogen Peroxide 30%:
Stability: Stable.
Incompatibilities: Reducing agents, organic, combustible materials, metal
powders, strong alkalis.
Storage conditions: Store loosly capped in the dark at room temperature.
Hazardous decomposition products: Oxygen
Hazardous polymerization: Will not occur.
Phosphate Buffered Saline:
Stability: Stable.
Incompatibilities: Oxidizers, acids
Storage conditions: Store tightly capped in the dark at room temperature.
Hazardous decomposition products: Phosphor Oxides and Sodium compounds.
Hazardous polymerization: Will not occur.
Msds21026 8-OHdG ELISA Assay Kit.doc
OXIS International, Inc.
MATERIAL SAFETY DATA SHEET (page 5 of 7)
Phosphoric Acid:
Stability: Stable.
Incompatibilities: Alkalis, metals, aldehydes, cyanide compounds, mercaptans,
sulfides, epoxides, azo compounds, polymerizable
compounds
Storage conditions: Hygroscopic. Store tightly capped in the dark at room
temperature.
Hazardous decomposition products: N/A
Hazardous polymerization: Will not occur.
Polyoxyethylene (20) Sorbitan Monolaurate:
Stability: Stable.
Incompatibilities: Strong acids
Storage conditions: Store tightly capped.
Hazardous decomposition products: Not known
Hazardous polymerization: Will not occur.
Sodium Azide:
Stability: Stable.
Incompatibilities: Strong oxidizers, acids, heavy metals, almunium,
dibromomalononitrile, nitric acid, dimethyl sulfate, benzyl
chloride, potassium hydroxide, bromine, carbon disulfate,
chromyl chloride.
Storage conditions: Store tightly capped in the dark at room temperature.
Hazardous decomposition products: Nitrogen oxides, nitrogen gas and sodium compounds.
Hazardous polymerization: Will not occur.
SECTION V - HEALTH HAZARD
3,3',5,5'-Tetramethylbenzidine (TMB):
Threshold limit value: N/A
Toxicity data: ORL-QAL LD50: >316 MG/KG.
The toxicological properties of this material have not been
thoroughly investigated.
Health hazards: May be harmful by inhalation, ingestion, or skin absorption.
Causes skin irritation. may be harmful if absorbed through
the skin. Causes eye irritation. Irritating to mucous
membranes and upper respiratory tract. May be harmful if
inhaled. May be harmful if swallowed.
First aid procedures: If swallowed seek immediate medical attention. In case of
contact with eyes or skin, flush with plenty of water and seek
medical attention. If inhaled, expose to fresh air and seek
medical attention.
8-Hydroxy-2'-deoxyguanosine:
Threshold limit value: None established
Toxicity data: The toxicological properties of this compound have not been
fully investigated.
Health hazards: Iritating to skin, eyes, nose, throat and respiratory tract.
First aid procedures: If swallowed seek immediate medical attention. In case of
contact with eyes or skin, flush with plenty of water and seek
medical attention. If inhaled, expose to fresh air and seek
medical attention.
Msds21026 8-OHdG ELISA Assay Kit.doc
OXIS International, Inc.
MATERIAL SAFETY DATA SHEET (page 6 of 7)
Citrate-Phosphate Buffered Saline:
Threshold limit value: None established.
Toxicity data: Irritant
Health hazards: May be harmful by inhalation, ingestion, or skin absorption.
Causes skin and eye irritation. Material is irritating to
mucous membranes and upper respiratory tract.
First aid procedures: If swallowed, wash out mouth with water provided person is
conscious; seek immediate medical attention. In case of
contact with eyes or skin, flush with plenty of water and seek
medical attention. If inhaled, expose to fresh air and seek
medical attention.
Hydrogen Peroxide (30%):
3
Threshold limit value: 1 ppm, 1.4 mg/m
Toxicity data: Harmful. Oxidizer. Corrosive.
Health hazards: May be harmful by inhalation, ingestion, or skin absorption.
First aid procedures: If swallowed seek immediate medical attention. In case of
contact with eyes or skin, flush with plenty of water and seek
medical attention. If inhaled, expose to fresh air and seek
medical attention.
Phosphoric Acid:
3
Threshold limit value: 1 mg/m
Toxicity data: Reactive. Corrosive. Irritant.
Health hazards: May be harmful by inhalation, ingestion, or skin absorption.
First aid procedures: If swallowed seek immediate medical attention. In case of
contact with eyes or skin, flush with plenty of water and seek
medical attention. If inhaled, expose to fresh air and seek
medical attention.
Phosphate Buffered Saline:
Threshold limit value: None established.
Toxicity data: Irritant
Health hazards: May be harmful by inhalation, ingestion, or skin absorption.
Causes skin and eye irritation. Material is irritating to
mucous membranes and upper respiratory tract.
First aid procedures: If swallowed, wash out mouth with water provided person is
conscious; seek immediate medical attention. In case of
contact with eyes or skin, flush with plenty of water and seek
medical attention. If inhaled, expose to fresh air and seek
medical attention.
Polyoxyethylene (20) Sorbitan Monolaurate:
Threshold limit value: None established.
Toxicity data: Irritant
Health hazards: May be harmful by inhalation or ingestion.
First aid procedures: If swallowed seek immediate medical attention. In case of
contact with eyes or skin, flush with plenty of water and seek
medical attention. If inhaled, expose to fresh air and seek
medical attention.
Sodium Azide:
Threshold limit value: None established.
Toxicity data: Toxic.
Health hazards: May be fatal by inhalation or ingestion. May be harmful if
absorbed.
First aid procedures: If swallowed seek immediate medical attention. In case of
contact with eyes or skin, flush with plenty of water and seek
medical attention. If inhaled, expose to fresh air and seek
medical attention.
Msds21026 8-OHdG ELISA Assay Kit.doc
OXIS International, Inc.
MATERIAL SAFETY DATA SHEET (page 7 of 7)
SECTION VI - SPECIAL PROTECTION INFORMATION
Respiratory protection: Approved respirator
Ventilation: Required
Mechanical: Required
Local Exhaust: N/A
Other protective clothing or equipment: Laboratory coats, gloves and eye protection recommended.
SECTION VII - SPECIAL PRECAUTIONS AND SPILL/LEAK PROCEDURES
Steps to be taken in case material is released or spilled:
3,3',5,5'-Tetramethylbenzidine (TMB):
Wear protective clothing (laboratory coats, gloves and eye
protection), wipe up spill with absorbant material and dispose
in appropriate container.
8-Hydroxy-2'-deoxyguanosine:
Wear protective clothing (laboratory coats, gloves and eye
protection), wipe up spill with absorbant material and dispose
in appropriate container.
Citrate-Phosphate Buffered Saline:
Wear protective clothing (laboratory coats, gloves and eye
protection), wipe up spill with absorbant material and dispose
in appropriate container. After clean-up, wash the spill site
with with soap and water and ventilate.
Hydrogen Peroxide 30%:
Wear protective clothing (laboratory coats, gloves and eye
protection), wipe up spill with absorbant material and dispose
in appropriate container.
Phosphate Buffered Saline:
Wear protective clothing (laboratory coats, gloves and eye
protection), wipe up spill with absorbant material and dispose
in appropriate container. After clean-up, wash the spill site
with with soap and water and ventilate.
Phosphoric Acid:
Wear protective clothing (laboratory coats, gloves and eye
protection), dilute with plenty of water and neturalize with
soda ash. Wipe up spill with absorbant material, and
dispose in appropriate container. After clean-up, wash the
spill site with with soap and water and ventilate.
Polyoxyethylene (20) Sorbitan Monolaurate:
Remove ignition sources and ventilate area. If unable to
ventilate, wear suitable respiratory equipment. Wear
protective clothing (laboratory coats, gloves and eye
protection), wipe up spill with absorbant material and dispose
in appropriate container. After clean-up, wash the spill site
with with soap and water and ventilate.
Sodium Azide:
Wear protective clothing (laboratory coats, gloves and eye
protection), wipe up spill with absorbant material and dispose
in appropriate container.
Clean-up of spill site: Wash the spill site with water.
Waste disposal methods: Dispose of waste in accordance with all Federal, State and
local regulations.
The information contained in this MSDS is believed to be correct to the best of our knowledge. However, we make no warranty,
expressed or implied, with respect to such information. The information may not be all inclusive, the user should make an
independent determination of the suitability of the information based on all sources available and adopt appropriate safety
precautions. OXIS International, Inc. shall not be held liable for any damage resulting from contact or handling of this product.
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Msds21026 8-OHdG ELISA Assay Kit.doc
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