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 Nigrosin Solution

Section 1 - Chemical Product and Company Identification

MSDS Name: Nigrosin Solution
Catalog Numbers: S80108
Nigrosine biological stain
Company Identification:

              Fisher Scientific
              1 Reagent Lane
              Fair Lawn, NJ 07410
For information, call:
Emergency Number:
For CHEMTREC assistance, call:
For International CHEMTREC assistance, call:

Section 2 - Composition, Information on Ingredients

CAS# Chemical Name Percent EINECS/ELINCS
7732-18-5 Water 97.0 231-791-2
8005-03-6 Nigrosine 3.0 unlisted

Section 3 - Hazards Identification


Appearance: dark blue-violet liquid.
Caution! May cause irritation. The toxicological properties of this material have not been fully investigated.
Target Organs: None.

Potential Health Effects
Eye: May cause eye irritation.
Skin: May cause skin irritation. Low hazard for usual industrial handling.
Ingestion: May cause gastrointestinal irritation with nausea, vomiting and diarrhea. The toxicological properties of this substance have not been fully investigated.
Inhalation: May cause respiratory tract irritation. The toxicological properties of this substance have not been fully investigated.
Chronic: Not available.

Section 4 - First Aid Measures

Eyes: Get medical aid. Gently lift eyelids and flush continuously with wate r.
Skin: Get medical aid if irritation develops or persists. Flush skin with plenty of soap and water.
Ingestion: If victim is conscious and alert, give 2-4 cupfuls of milk or water. Get medical aid.
Inhalation: Remove from exposure and move to fresh air immediately. Get medical aid if cough or other symptoms appear.
Notes to Physician: Treat symptomatically and supportively.
Antidote: None reported.

Section 5 - Fire Fighting Measures

General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear.
Extinguishing Media: Substance is noncombustible; use agent most appropriate to extinguish surrounding fire.
Flash Point: Not applicable.
Autoignition Temperature: Not applicable.
Explosion Limits, Lower:Not available.
Upper: Not available.
NFPA Rating: (estimated) Health: 1; Flammability: 0; Instability: 0

Section 6 - Accidental Release Measures

General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Absorb spill with inert material (e.g. vermiculite, sand or earth), then place in suitable container.

Section 7 - Handling and Storage

Handling: Use with adequate ventilation. Avoid contact with eyes. Keep container tightly closed.
Storage: Store in a cool, dry place.

Section 8 - Exposure Controls, Personal Protection

Engineering Controls: Good general ventilation should be sufficient to control airborne levels.
Exposure Limits
Chemical Name ACGIH NIOSH OSHA - Final PELs
Water none listed none listed none listed
Nigrosine none listed none listed none listed

OSHA Vacated PELs: Water: No OSHA Vacated PELs are listed for this chemical. Nigrosine: No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Wear appropriate gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to minimize contact with skin.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

Section 9 - Physical and Chemical Properties

Physical State: Liquid
Appearance: dark blue-violet
Odor: odorless
pH: Not available.
Vapor Pressure: 14 mm Hg @16 deg C
Vapor Density: 0.7 (water)
Evaporation Rate:>1.0 (ether=1)
Viscosity: Not available.
Boiling Point: 100 deg C
Freezing/Melting Point:0 deg C
Decomposition Temperature:Not available.
Solubility: Completely soluble in water.
Specific Gravity/Density:~1.0
Molecular Formula:solution
Molecular Weight:Not available.

Section 10 - Stability and Reactivity

Chemical Stability: Stable.
Conditions to Avoid: Strong oxidants.
Incompatibilities with Other Materials: Strong oxidizers.
Hazardous Decomposition Products: Irritating and toxic gases.
Hazardous Polymerization: Has not been reported.

Section 11 - Toxicological Information

CAS# 7732-18-5: ZC0110000      
CAS# 8005-03-6: GC4762250
CAS# 7732-18-5:
     Oral, rat: LD50 = >90 mL/kg;

CAS# 8005-03-6:

CAS# 7732-18-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 8005-03-6: Not listed by ACGIH, IARC, NTP, or CA Prop 65.

Epidemiology: No data available.
Teratogenicity: No data available.
Reproductive Effects: No data available.
Mutagenicity: No data available.
Neurotoxicity: No data available.
Other Studies:

Section 12 - Ecological Information

Ecotoxicity: No data available. No information available.
Environmental: No information reported.
Physical: No information available.
Other: None.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series: None listed.

Section 14 - Transport Information

Shipping Name: Not regulated as a hazardous material No information available.
Hazard Class:
UN Number:
Packing Group:

Section 15 - Regulatory Information


     CAS# 7732-18-5 is listed on the TSCA inventory.
     CAS# 8005-03-6 is listed on the TSCA inventory.
Health & Safety Reporting List
     None of the chemicals are on the Health & Safety Reporting List.
Chemical Test Rules
     None of the chemicals in this product are under a Chemical Test Rule.
Section 12b
     None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
     None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
     None of the chemicals in this material have an RQ.
SARA Section 302 Extremely Hazardous Substances
     None of the chemicals in this product have a TPQ.
Section 313      No chemicals are reportable under Section 313.
Clean Air Act:
     This material does not contain any hazardous air pollutants.
     This material does not contain any Class 1 Ozone depletors.
     This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
     None of the chemicals in this product are listed as Hazardous Substances under the CWA.
     None of the chemicals in this product are listed as Priority Pollutants under the CWA.
     None of the chemicals in this product are listed as Toxic Pollutants under the CWA.
     None of the chemicals in this product are considered highly hazardous by OSHA.
     CAS# 7732-18-5 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
     CAS# 8005-03-6 is not present on state lists from CA, PA, MN, MA, FL, or NJ.

California Prop 65

California No Significant Risk Level: None of the chemicals in this product are listed.

European/International Regulations

European Labeling in Accordance with EC Directives
Hazard Symbols:
      Not available.
Risk Phrases:

Safety Phrases:

WGK (Water Danger/Protection)

     CAS# 7732-18-5: No information available.
     CAS# 8005-03-6: No information available.
Canada - DSL/NDSL
     CAS# 7732-18-5 is listed on Canada's DSL List.
     CAS# 8005-03-6 is listed on Canada's DSL List.
Canada - WHMIS
     not available.
This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations.
Canadian Ingredient Disclosure List

Section 16 - Additional Information

MSDS Creation Date: 9/02/1997
Revision #5 Date: 11/07/2007

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.

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