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MSDS  
:
 Decolorizing Solution. Component of Gram Stain Kits with stabilized and nonstabilized iodine.
CAS  
:
 67-64-1
SYNONYMS  
:
 None.

Section 1 - Chemical Product and Company Identification
MSDS Name: Stabilized Iodine
Catalog Numbers: 23-005-84, 23-005-85, 2300585B, 281407B, 66766
Synonyms:
Mixture
Company Identification:

              Fisher Diagnostics
              Fisher Scientific Company, LLC
              8365 Valley Pike
              Middletown, VA 22645-0307

For information, call:
800-524-0294
Emergency Number:
800-524-0294
For CHEMTREC assistance, call:
800-424-9300
For International CHEMTREC assistance, call:
703-527-3887

Section 2 - Composition, Information on Ingredients

CAS# Chemical Name Percent EINECS/ELINCS
25655-41-8 Poviodine-Iodine 12.57 unlisted
7681-11-0 Potassium iodide <2.0 231-659-4
7732-18-5 DI Water Balance 231-791-2

Section 3 - Hazards Identification

EMERGENCY OVERVIEW

Appearance: Dark, reddish-brown.
Caution! May cause allergic skin reaction. Causes eye irritation. Causes digestive and respiratory tract irritation. May cause skin irritation. May cause fetal effects based upon animal studies.
Target Organs: None.


Potential Health Effects
Eye: Causes eye irritation. May cause eye injury.
Skin: Causes skin irritation. May cause skin sensitization, an allergic reaction, which becomes evident upon re-exposure to this material.
Ingestion: Causes gastrointestinal irritation with nausea, vomiting and diarrhea.
Inhalation: Causes respiratory tract irritation.
Chronic: May cause fetal effects.

Section 4 - First Aid Measures


Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid imme diately.
Skin: Get medical aid if irritation develops or persists. Wash clothing before reuse. Flush skin with plenty of soap and water.
Ingestion: If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid immediately.
Inhalation: Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid if cough or other symptoms appear.
Notes to Physician: Treat symptomatically and supportively.

Section 5 - Fire Fighting Measures


General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear.
Extinguishing Media: Use water spray, dry chemical, carbon dioxide, or chemical foam.
Flash Point: Not available.
Autoignition Temperature: Not available.
Explosion Limits, Lower:N/A
Upper: N/A
NFPA Rating: (estimated) Health: 1; Flammability: 0; Instability: 0

Section 6 - Accidental Release Measures


General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Absorb spill with inert material (e.g. vermiculite, sand or earth), then place in suitable container. Clean up spills immediately, observing precautions in the Protective Equipment section. Provide ventilation.

Section 7 - Handling and Storage


Handling: Wash thoroughly after handling. Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation.
Storage: Store in a cool, dry, well-ventilated area away from incompatible substances. Keep containers tightly closed.

Section 8 - Exposure Controls, Personal Protection


Engineering Controls: Good general ventilation should be sufficient to control airborne levels.
Exposure Limits
Chemical Name ACGIH NIOSH OSHA - Final PELs
Poviodine-Iodine none listed none listed none listed
Potassium iodide none listed none listed none listed
DI Water none listed none listed none listed

OSHA Vacated PELs: Poviodine-Iodine: No OSHA Vacated PELs are listed for this chemical. Potassium iodide: No OSHA Vacated PELs are listed for this chemical. DI Water: No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Wear appropriate protective gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

Section 9 - Physical and Chemical Properties


Physical State: Liquid
Appearance: Dark, reddish-brown
Odor: Iodine
pH: Not available.
Vapor Pressure: Not available.
Vapor Density: Not available.
Evaporation Rate:Not available.
Viscosity: Not available.
Boiling Point: Not available.
Freezing/Melting Point:Not available.
Decomposition Temperature:Not available.
Solubility: Soluble in water.
Specific Gravity/Density:~1
Molecular Formula:Not available.
Molecular Weight:Not available.

Section 10 - Stability and Reactivity


Chemical Stability: Stable under normal temperatures and pressures.
Conditions to Avoid: Incompatible materials, excess heat.
Incompatibilities with Other Materials: Incompatable with potassium iodide include: salts of alkaloids, chloral hydrate, calomel(mercurous chloride) potassium chlorate, metallic salts, tartaric and other acids, bromine trifluoride, and fluorine perchlorate, oxidizing agents. Incompatibilities with PVP include oxidizing agents.
Hazardous Decomposition Products: Irritating and toxic fumes and gases, oxides of potassium, iodine.
Hazardous Polymerization: Will not occur.

Section 11 - Toxicological Information


RTECS#:      
CAS# 25655-41-8: TR1579600      
CAS# 7681-11-0: TT2975000      
CAS# 7732-18-5: ZC0110000
LD50/LC50:
CAS# 25655-41-8:
     Draize test, rabbit, skin: 500 mg Mild;
     Oral, mouse: LD50 = 8100 mg/kg;
     Oral, rat: LD50 = >8 gm/kg;
.

CAS# 7681-11-0:
.

CAS# 7732-18-5:
     Oral, rat: LD50 = >90 mL/kg;
.

Carcinogenicity:
CAS# 25655-41-8: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 7681-11-0: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 7732-18-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.

Epidemiology: No information available.
Teratogenicity: Iodine salts can produce deformity, illness, and death of a fetus. PVP(iodine complex) has been shown to present a possible risk of harm to the unborn child.
Reproductive Effects: PVP(iodine complex) has been shown to produce reproductive effects when there is overexposure.
Mutagenicity: No information available.
Neurotoxicity: No information available.
Other Studies:

Section 12 - Ecological Information


Ecotoxicity: No data available. No information available.
Environmental: No information found.
Physical: No information found.
Other: No information available.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series: None listed.

Section 14 - Transport Information

US DOT Canada TDG
Shipping Name: Not regulated as a hazardous material No information available.
Hazard Class:
UN Number:
Packing Group:

Section 15 - Regulatory Information

US FEDERAL

TSCA
     CAS# 25655-41-8 is listed on the TSCA inventory.
     CAS# 7681-11-0 is listed on the TSCA inventory.
     CAS# 7732-18-5 is listed on the TSCA inventory.
Health & Safety Reporting List
     None of the chemicals are on the Health & Safety Reporting List.
Chemical Test Rules
     None of the chemicals in this product are under a Chemical Test Rule.
Section 12b
     None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
     None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
     None of the chemicals in this material have an RQ.
SARA Section 302 Extremely Hazardous Substances
     None of the chemicals in this product have a TPQ.
SARA Codes
     CAS # 7681-11-0: immediate, delayed.
Section 313      No chemicals are reportable under Section 313.
Clean Air Act:
     This material does not contain any hazardous air pollutants.
     This material does not contain any Class 1 Ozone depletors.
     This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
     None of the chemicals in this product are listed as Hazardous Substances under the CWA.
     None of the chemicals in this product are listed as Priority Pollutants under the CWA.
     None of the chemicals in this product are listed as Toxic Pollutants under the CWA.
OSHA:
     None of the chemicals in this product are considered highly hazardous by OSHA.
STATE
     CAS# 25655-41-8 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
     CAS# 7681-11-0 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
     CAS# 7732-18-5 is not present on state lists from CA, PA, MN, MA, FL, or NJ.

California Prop 65

California No Significant Risk Level: None of the chemicals in this product are listed.

European/International Regulations

European Labeling in Accordance with EC Directives
Hazard Symbols:
      Not available.
Risk Phrases:


Safety Phrases:


WGK (Water Danger/Protection)

     CAS# 25655-41-8: 2
     CAS# 7681-11-0: 1
     CAS# 7732-18-5: No information available.
Canada - DSL/NDSL
     CAS# 25655-41-8 is listed on Canada's DSL List.
     CAS# 7681-11-0 is listed on Canada's DSL List.
     CAS# 7732-18-5 is listed on Canada's DSL List.
Canada - WHMIS
     WHMIS: Not available.
This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations.
Canadian Ingredient Disclosure List
     CAS# 7681-11-0 is listed on the Canadian Ingredient Disclosure List.

Section 16 - Additional Information

MSDS Creation Date: 4/16/1999
Revision #4 Date: 10/03/2005

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.

Section 1 - Chemical Product and Company Identification
MSDS Name: Safranin Stain
Catalog Numbers: 23255963, 23270183, 23281407D, 23291476, 66762, 66762S, 66770B, 66780B, 66782
Synonyms:
Mixture
Company Identification:

              Fisher Diagnostics
              Fisher Scientific Company, LLC
              8365 Valley Pike
              Middletown, VA 22645-0307

For information, call:
800-524-0294
Emergency Number:
800-524-0294
For CHEMTREC assistance, call:
800-424-9300
For International CHEMTREC assistance, call:
703-527-3887

Section 2 - Composition, Information on Ingredients

CAS# Chemical Name Percent EINECS/ELINCS
7732-18-5 Water <80 231-791-2
64-17-5 Ethyl alcohol 19 200-578-6
67-56-1 Methyl alcohol 1 200-659-6
477-73-6 Safranin 0.604 207-518-8

Section 3 - Hazards Identification

EMERGENCY OVERVIEW

Appearance: red liquid. Flash Point: 82 deg F.
Warning! Causes eye irritation. Flammable liquid and vapor. May be absorbed through intact skin. May cause skin and respiratory tract irritation. May cause central nervous system depression. May cause liver and kidney damage. May cause blindness if swallowed.
Target Organs: Central nervous system, liver, eyes.


Potential Health Effects
Eye: Produces irritation, characterized by a burning sensation, redness, tearing, inflammation, and possible corneal injury. Vapors may cause eye irritation. May cause painful sensitization to light.
Skin: May cause skin irritation. May be absorbed through the skin in harmful amounts.
Ingestion: May cause systemic toxicity with acidosis. May cause liver and kidney damage. May cause central nervous system depression, characterized by excitement, followed by headache, dizziness, drowsiness, and nausea. Advanced stages may cause collapse, unconsciousness, coma and possible death due to respiratory failure. May cause blindness if swallowed.
Inhalation: May cause respiratory tract irritation. May cause liver and kidney damage. May cause narcotic effects in high concentration. May cause drowsiness, unconsciousness, and central nervous system depression.
Chronic: Prolonged or repeated skin contact may cause dermatitis. Chronic inhalation may cause effects similar to those of acute inhalation.

Section 4 - First Aid Measures


Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid imme diately.
Skin: Get medical aid. Rinse area with large amounts of water for at least 15 minutes.
Ingestion: Call a poison control center. If swallowed, do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical aid.
Inhalation: Get medical aid immediately. Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen.
Notes to Physician: Treat symptomatically and supportively.

Section 5 - Fire Fighting Measures


General Information: Containers can build up pressure if exposed to heat and/or fire. As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Use water spray to keep fire-exposed containers cool. Vapors may be heavier than air. They can spread along the ground and collect in low or confined areas. Containers may explode when heated. Runoff from fire control or dilution water may cause pollution. Flammable liquid and vapor.
Extinguishing Media: Use water spray to cool fire-exposed containers. Use water spray, dry chemical, carbon dioxide, or chemical foam. Do NOT use straight streams of water. Cool containers with flooding quantities of water until well after fire is out.
Flash Point: 82e deg F ( 27.78 deg C)
Autoignition Temperature: Not available.
Explosion Limits, Lower:3.3
Upper: 19
NFPA Rating: (estimated) Health: 2; Flammability: 3; Instability: 0

Section 6 - Accidental Release Measures


General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Absorb spill with inert material (e.g. vermiculite, sand or earth), then place in suitable container. Remove all sources of ignition. Provide ventilation.

Section 7 - Handling and Storage


Handling: Wash thoroughly after handling. Avoid contact with eyes, skin, and clothing. Empty containers retain product residue, (liquid and/or vapor), and can be dangerous. Keep container tightly closed. Do not pressurize, cut, weld, braze, solder, drill, grind, or expose empty containers to heat, sparks or open flames. Use only with adequate ventilation. Keep away from heat, sparks and flame. Avoid breathing vapor or mist.
Storage: Keep away from sources of ignition. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances. Flammables-area.

Section 8 - Exposure Controls, Personal Protection


Engineering Controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate general or local exhaust ventilation to keep airborne concentrations below the permissible exposure limits.
Exposure Limits
Chemical Name ACGIH NIOSH OSHA - Final PELs
Water none listed none listed none listed
Ethyl alcohol 1000 ppm TWA 1000 ppm TWA; 1900 mg/m3 TWA 3300 ppm IDLH 1000 ppm TWA; 1900 mg/m3 TWA
Methyl alcohol 200 ppm TWA; 250 ppm STEL; Skin - potential significant contribution to overall exposure by the cutaneous r oute 200 ppm TWA; 260 mg/m3 TWA 6000 ppm IDLH 200 ppm TWA; 260 mg/m3 TWA
Safranin none listed none listed none listed

OSHA Vacated PELs: Water: No OSHA Vacated PELs are listed for this chemical. Ethyl alcohol: 1000 ppm TWA; 1900 mg/m3 TWA Methyl alcohol: 200 ppm TWA; 260 mg/m3 TWA Safranin: No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear chemical splash goggles.
Skin: Wear appropriate protective gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

Section 9 - Physical and Chemical Properties


Physical State: Liquid
Appearance: red
Odor: pungent odor
pH: Not available.
Vapor Pressure: 40 mm Hg @19 deg C
Vapor Density: 1.59 (air=1)
Evaporation Rate:Not available.
Viscosity: Not available.
Boiling Point: 95 deg C
Freezing/Melting Point:Not available.
Decomposition Temperature:Not available.
Solubility: Soluble.
Specific Gravity/Density:1
Molecular Formula:Not available.
Molecular Weight:Not available.

Section 10 - Stability and Reactivity


Chemical Stability: Stable under normal temperatures and pressures.
Conditions to Avoid: Ignition sources, excess heat.
Incompatibilities with Other Materials: Ethanol is incompatible with acetic anhydride, acetyl bromide, ammonia + silver nitrate, disulfuric acid + nitric acid, dichloromethane + sulfuric acid, disulfuryl difluoride, magnesium perchlorate, nitric acid + silver, oxidants, phosphorus (III) oxide, platinum, potassium, potassium tert-butoxide, silver nitrate, silver oxide, sodium and tetrachlorosilane. Methanol is incompatible with acetyl bromide, alkylaluminum solutions, beryllium hydride, carbon tetrachloride + metals, chloroform + sodium, chloroform + sodium hydroxide, cyanuric chloride, dichloromethane, diethylzinc, metals, oxidants, phosphorus (III) oxide, and potassium tert-butoxide.
Hazardous Decomposition Products: Hydrogen chloride, carbon monoxide, oxides of nitrogen, carbon dioxide, formaldehyde.
Hazardous Polymerization: Will not occur.

Section 11 - Toxicological Information


RTECS#:      
CAS# 7732-18-5: ZC0110000      
CAS# 64-17-5: KQ6300000      
CAS# 67-56-1: PC1400000      
CAS# 477-73-6: SG1623000
LD50/LC50:
CAS# 7732-18-5:
     Oral, rat: LD50 = >90 mL/kg;
.

CAS# 64-17-5:
     Draize test, rabbit, eye: 500 mg Severe;
     Draize test, rabbit, eye: 500 mg/24H Mild;
     Draize test, rabbit, skin: 20 mg/24H Moderate;
     Inhalation, mouse: LC50 = 39 gm/m3/4H;
     Inhalation, rat: LC50 = 20000 ppm/10H;
     Oral, mouse: LD50 = 3450 mg/kg;
     Oral, rabbit: LD50 = 6300 mg/kg;
     Oral, rat: LD50 = 7060 mg/kg;
     Oral, rat: LD50 = 9000 mg/kg;
.

CAS# 67-56-1:
     Draize test, rabbit, eye: 40 mg Moderate;
     Draize test, rabbit, eye: 100 mg/24H Moderate;
     Draize test, rabbit, skin: 20 mg/24H Moderate;
     Inhalation, rabbit: LC50 = 81000 mg/m3/14H;
     Inhalation, rat: LC50 = 64000 ppm/4H;
     Oral, mouse: LD50 = 7300 mg/kg;
     Oral, rabbit: LD50 = 14200 mg/kg;
     Oral, rat: LD50 = 5600 mg/kg;
     Skin, rabbit: LD50 = 15800 mg/kg;
.

CAS# 477-73-6:
.

Carcinogenicity:
CAS# 7732-18-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 64-17-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 67-56-1: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 477-73-6: Not listed by ACGIH, IARC, NTP, or CA Prop 65.

Epidemiology: This product contains methanol and ethanol which have been shown to produce fetoxicity in the embryo or fetus. Specific abnormalities for methanol include the cardiovascular, musculoskeletal and urogenital systems.
Teratogenicity: No information found
Reproductive Effects: Methanol has been shown to produce reproductive effects in laboratory animals. Prenatal exposure to ethanol is associated with a distinct pattern of congenital malformations that have been collectively termed the "fetal alcohol syndrome". Among the characteristics of this syndrome are intrauterine and postnatal growth deficiency, a distinctive pattern of physical malformation, and behavioral/cognitive impairment such as fine motor dysfunction and mental retardation. Not all affected children have all of the features of the syndrome.
Mutagenicity: Methanol has been shown to produce DNA damage in laboratory animals.
Neurotoxicity: No information found
Other Studies:

Section 12 - Ecological Information

No information available.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series:
CAS# 67-56-1: waste number U154 (Ignitable waste).

Section 14 - Transport Information

US DOT Canada TDG
Shipping Name: Flammable Liquid, N.O.S. No information available.
Hazard Class: 3
UN Number: UN1993
Packing Group: III

Section 15 - Regulatory Information

US FEDERAL

TSCA
     CAS# 7732-18-5 is listed on the TSCA inventory.
     CAS# 64-17-5 is listed on the TSCA inventory.
     CAS# 67-56-1 is listed on the TSCA inventory.
     CAS# 477-73-6 is listed on the TSCA inventory.
Health & Safety Reporting List
     None of the chemicals are on the Health & Safety Reporting List.
Chemical Test Rules
     None of the chemicals in this product are under a Chemical Test Rule.
Section 12b
     None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
     None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
     CAS# 67-56-1: 5000 lb final RQ; 2270 kg final RQ
SARA Section 302 Extremely Hazardous Substances
     None of the chemicals in this product have a TPQ.
SARA Codes
     CAS # 64-17-5: immediate, delayed, fire.
     CAS # 67-56-1: immediate, fire.
     CAS # 477-73-6: immediate, reactive.
Section 313
     Methyl alcohol is not at a high enough concentration to be reportable under Section 313.      No chemicals are reportable under Section 313.
Clean Air Act:
     CAS# 67-56-1 is listed as a hazardous air pollutant (HAP).

     This material does not contain any Class 1 Ozone depletors.
     This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
     None of the chemicals in this product are listed as Hazardous Substances under the CWA.
     None of the chemicals in this product are listed as Priority Pollutants under the CWA.
     None of the chemicals in this product are listed as Toxic Pollutants under the CWA.
OSHA:
     None of the chemicals in this product are considered highly hazardous by OSHA.
STATE
     CAS# 7732-18-5 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
     CAS# 64-17-5 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 67-56-1 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 477-73-6 is not present on state lists from CA, PA, MN, MA, FL, or NJ.

California Prop 65
WARNING: This product contains Ethyl alcohol, a chemical known to the state of California to cause developmental reproductive toxicity.
California No Significant Risk Level: None of the chemicals in this product are listed.

European/International Regulations

European Labeling in Accordance with EC Directives
Hazard Symbols:
      Not available.
Risk Phrases:


Safety Phrases:


WGK (Water Danger/Protection)

     CAS# 7732-18-5: No information available.
     CAS# 64-17-5: 0
     CAS# 67-56-1: 1
     CAS# 477-73-6: No information available.
Canada - DSL/NDSL
     CAS# 7732-18-5 is listed on Canada's DSL List.
     CAS# 64-17-5 is listed on Canada's DSL List.
     CAS# 67-56-1 is listed on Canada's DSL List.
     CAS# 477-73-6 is listed on Canada's DSL List.
Canada - WHMIS
     This product has a WHMIS classification of B2.
This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations.
Canadian Ingredient Disclosure List
     CAS# 64-17-5 is listed on the Canadian Ingredient Disclosure List.
     CAS# 67-56-1 is listed on the Canadian Ingredient Disclosure List.

Section 16 - Additional Information

MSDS Creation Date: 4/16/1999
Revision #9 Date: 7/23/2003

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.

Section 1 - Chemical Product and Company Identification
MSDS Name: Gram Safranin
Catalog Numbers: 66760B
Synonyms:
Mixture
Company Identification:

              Fisher Diagnostics
              Fisher Scientific Company, LLC
              8365 Valley Pike
              Middletown, VA 22645-0307

For information, call:
800-524-0294
Emergency Number:
800-524-0294
For CHEMTREC assistance, call:
800-424-9300
For International CHEMTREC assistance, call:
703-527-3887

Section 2 - Composition, Information on Ingredients

CAS# Chemical Name Percent EINECS/ELINCS
7732-18-5 Deionized Water 79.3 231-791-2
64-17-5 Ethyl Alcohol 19 200-578-6
67-56-1 Methyl Alcohol 1 200-659-6
477-73-6 Safranin <0.7 207-518-8

Section 3 - Hazards Identification

EMERGENCY OVERVIEW

Appearance: red liquid. Flash Point: 82 deg F.
Caution! May cause severe eye irritation and possible injury. May be absorbed through intact skin. Combustible liquid and vapor. May cause respiratory tract irritation. May cause skin irritation. May cause central nervous system depression. May cause liver and kidney damage. May cause blindness if swallowed. May cause fetal effects based upon animal studies.
Target Organs: Kidneys, central nervous system, liver, eyes.


Potential Health Effects
Eye: Produces irritation, characterized by a burning sensation, redness, tearing, inflammation, and possible corneal injury. Vapors may cause eye irritation. May cause painful sensitization to light.
Skin: May cause skin irritation. May be absorbed through the skin in harmful amounts.
Ingestion: May cause kidney damage. May cause systemic toxicity with acidosis. May cause liver and kidney damage. May cause central nervous system depression, characterized by excitement, followed by headache, dizziness, drowsiness, and nausea. Advanced stages may cause collapse, unconsciousness, coma and possible death due to respiratory failure. May cause blindness if swallowed.
Inhalation: Harmful if inhaled. May cause respiratory tract irritation. May cause liver and kidney damage. May cause narcotic effects in high concentration. May cause drowsiness, unconsciousness, and central nervous system depression.
Chronic: Prolonged or repeated skin contact may cause dermatitis. Chronic inhalation may cause effects similar to those of acute inhalation.

Section 4 - First Aid Measures


Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid imme diately.
Skin: Get medical aid. Rinse area with large amounts of water for at least 15 minutes.
Ingestion: Call a poison control center. If swallowed, do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical aid.
Inhalation: Get medical aid immediately. Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen.
Notes to Physician: Treat symptomatically and supportively.

Section 5 - Fire Fighting Measures


General Information: Containers can build up pressure if exposed to heat and/or fire. As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Use water spray to keep fire-exposed containers cool. Combustible liquid. Containers may explode when heated.
Extinguishing Media: Use water spray to cool fire-exposed containers. Use water spray, dry chemical, carbon dioxide, or chemical foam. Do NOT use straight streams of water. Cool containers with flooding quantities of water until well after fire is out.
Flash Point: 82e deg F ( 27.78 deg C)
Autoignition Temperature: Not available.
Explosion Limits, Lower:3.3
Upper: 19
NFPA Rating: (estimated) Health: 1; Flammability: 2; Instability: 0

Section 6 - Accidental Release Measures


General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Sweep up, then place into a suitable container for disposal. Remove all sources of ignition. Absorb spill using an absorbent, non-combustible material such as earth, sand, or vermiculite. Do not use combustible materials such as sawdust. Provide ventilation.

Section 7 - Handling and Storage


Handling: Wash thoroughly after handling. Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Empty containers retain product residue, (liquid and/or vapor), and can be dangerous. Keep container tightly closed. Keep away from heat, sparks and flame. Avoid ingestion and inhalation. Do not pressurize, cut, weld, braze, solder, drill, grind, or expose empty containers to heat, sparks or open flames.
Storage: Keep away from heat, sparks, and flame. Keep away from sources of ignition. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.

Section 8 - Exposure Controls, Personal Protection


Engineering Controls: Use adequate general or local exhaust ventilation to keep airborne concentrations below the permissible exposure limits.
Exposure Limits
Chemical Name ACGIH NIOSH OSHA - Final PELs
Deionized Water none listed none listed none listed
Ethyl Alcohol 1000 ppm TWA 1000 ppm TWA; 1900 mg/m3 TWA 3300 ppm IDLH 1000 ppm TWA; 1900 mg/m3 TWA
Methyl Alcohol 200 ppm TWA; 250 ppm STEL; Skin - potential significant contribution to overall exposure by the cutaneous r oute 200 ppm TWA; 260 mg/m3 TWA 6000 ppm IDLH 200 ppm TWA; 260 mg/m3 TWA
Safranin none listed none listed none listed

OSHA Vacated PELs: Deionized Water: No OSHA Vacated PELs are listed for this chemical. Ethyl Alcohol: 1000 ppm TWA; 1900 mg/m3 TWA Methyl Alcohol: 200 ppm TWA; 260 mg/m3 TWA Safranin: No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Wear appropriate protective gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

Section 9 - Physical and Chemical Properties


Physical State: Liquid
Appearance: red
Odor: pungent odor
pH: Not available.
Vapor Pressure: 40 mm Hg @19C
Vapor Density: 1.59
Evaporation Rate:Not available.
Viscosity: Not available.
Boiling Point: 95 deg C
Freezing/Melting Point:Not available.
Decomposition Temperature:Not available.
Solubility: Soluble in water.
Specific Gravity/Density:1
Molecular Formula:Not available.
Molecular Weight:Not available.

Section 10 - Stability and Reactivity


Chemical Stability: Stable under normal temperatures and pressures.
Conditions to Avoid: Incompatible materials, ignition sources, excess heat.
Incompatibilities with Other Materials: Ethanol is incompatible with acetic anhydride, acetyl bromide, ammonia + silver nitrate, disulfuric acid + nitric acid, dichloromethane + sulfuric acid, disulfuryl difluoride, magnesium perchlorate, nitric acid + silver, oxidants, phosphorus (III) oxide, platinum, potassium, potassium tert-butoxide, silver nitrate, silver oxide, sodium and tetrachlorosilane. Methanol is incompatible with acetyl bromide, alkylaluminum solutions, beryllium hydride, carbon tetrachloride + metals, chloroform + sodium, chloroform + sodium hydroxide, cyanuric chloride, dichloromethane, diethylzinc, metals, oxidants, phosphorus (III) oxide, and potassium tert-butoxide.
Hazardous Decomposition Products: Hydrogen chloride, carbon monoxide, oxides of nitrogen, carbon dioxide, formaldehyde.
Hazardous Polymerization: Will not occur.

Section 11 - Toxicological Information


RTECS#:      
CAS# 7732-18-5: ZC0110000      
CAS# 64-17-5: KQ6300000      
CAS# 67-56-1: PC1400000      
CAS# 477-73-6: SG1623000
LD50/LC50:
CAS# 7732-18-5:
     Oral, rat: LD50 = >90 mL/kg;
.

CAS# 64-17-5:
     Draize test, rabbit, eye: 500 mg Severe;
     Draize test, rabbit, eye: 500 mg/24H Mild;
     Draize test, rabbit, skin: 20 mg/24H Moderate;
     Inhalation, mouse: LC50 = 39 gm/m3/4H;
     Inhalation, rat: LC50 = 20000 ppm/10H;
     Oral, mouse: LD50 = 3450 mg/kg;
     Oral, rabbit: LD50 = 6300 mg/kg;
     Oral, rat: LD50 = 7060 mg/kg;
     Oral, rat: LD50 = 9000 mg/kg;
.

CAS# 67-56-1:
     Draize test, rabbit, eye: 40 mg Moderate;
     Draize test, rabbit, eye: 100 mg/24H Moderate;
     Draize test, rabbit, skin: 20 mg/24H Moderate;
     Inhalation, rabbit: LC50 = 81000 mg/m3/14H;
     Inhalation, rat: LC50 = 64000 ppm/4H;
     Oral, mouse: LD50 = 7300 mg/kg;
     Oral, rabbit: LD50 = 14200 mg/kg;
     Oral, rat: LD50 = 5600 mg/kg;
     Skin, rabbit: LD50 = 15800 mg/kg;
.

CAS# 477-73-6:
.

Carcinogenicity:
CAS# 7732-18-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 64-17-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 67-56-1: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 477-73-6: Not listed by ACGIH, IARC, NTP, or CA Prop 65.

Epidemiology: This product contains methanol and ethanol which have been shown to produce fetoxicity in the embryo or fetus. Specific abnormalities for methanol include the cardiovascular, musculoskeletal and urogenital systems.
Teratogenicity: No data available.
Reproductive Effects: Methanol has been shown to produce reproductive effects in laboratory animals. Prenatal exposure to ethanol is associated with a distinct pattern of congenital malformations that have been collectively termed the "fetal alcohol syndrome". Among the characteristics of this syndrome are intrauterine and postnatal growth deficiency, a distinctive pattern of physical malformation, and behavioral/cognitive impairment such as fine motor dysfunction and mental retardation. Not all affected children have all of the features of the syndrome.
Mutagenicity: Methanol has been shown to produce DNA damage in laboratory animals.
Neurotoxicity: No data available.
Other Studies:

Section 12 - Ecological Information

No information available.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series:
CAS# 67-56-1: waste number U154 (Ignitable waste).

Section 14 - Transport Information

US DOT Canada TDG
Shipping Name: Not regulated as a hazardous material No information available.
Hazard Class:
UN Number:
Packing Group:

Section 15 - Regulatory Information

US FEDERAL

TSCA
     CAS# 7732-18-5 is listed on the TSCA inventory.
     CAS# 64-17-5 is listed on the TSCA inventory.
     CAS# 67-56-1 is listed on the TSCA inventory.
     CAS# 477-73-6 is listed on the TSCA inventory.
Health & Safety Reporting List
     None of the chemicals are on the Health & Safety Reporting List.
Chemical Test Rules
     None of the chemicals in this product are under a Chemical Test Rule.
Section 12b
     None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
     None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
     CAS# 67-56-1: 5000 lb final RQ; 2270 kg final RQ
SARA Section 302 Extremely Hazardous Substances
     None of the chemicals in this product have a TPQ.
SARA Codes
     CAS # 64-17-5: immediate, delayed, fire.
     CAS # 67-56-1: immediate, fire.
     CAS # 477-73-6: immediate, reactive.
Section 313
     Methyl Alcohol is not at a high enough concentration to be reportable under Section 313.      No chemicals are reportable under Section 313.
Clean Air Act:
     CAS# 67-56-1 is listed as a hazardous air pollutant (HAP).

     This material does not contain any Class 1 Ozone depletors.
     This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
     None of the chemicals in this product are listed as Hazardous Substances under the CWA.
     None of the chemicals in this product are listed as Priority Pollutants under the CWA.
     None of the chemicals in this product are listed as Toxic Pollutants under the CWA.
OSHA:
     None of the chemicals in this product are considered highly hazardous by OSHA.
STATE
     CAS# 7732-18-5 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
     CAS# 64-17-5 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 67-56-1 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 477-73-6 is not present on state lists from CA, PA, MN, MA, FL, or NJ.

California Prop 65
WARNING: This product contains Ethyl Alcohol, a chemical known to the state of California to cause developmental reproductive toxicity.
California No Significant Risk Level: None of the chemicals in this product are listed.

European/International Regulations

European Labeling in Accordance with EC Directives
Hazard Symbols:
      Not available.
Risk Phrases:


Safety Phrases:


WGK (Water Danger/Protection)

     CAS# 7732-18-5: No information available.
     CAS# 64-17-5: 0
     CAS# 67-56-1: 1
     CAS# 477-73-6: No information available.
Canada - DSL/NDSL
     CAS# 7732-18-5 is listed on Canada's DSL List.
     CAS# 64-17-5 is listed on Canada's DSL List.
     CAS# 67-56-1 is listed on Canada's DSL List.
     CAS# 477-73-6 is listed on Canada's DSL List.
Canada - WHMIS
     This product has a WHMIS classification of B3, D2A.
This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations.
Canadian Ingredient Disclosure List
     CAS# 64-17-5 is listed on the Canadian Ingredient Disclosure List.
     CAS# 67-56-1 is listed on the Canadian Ingredient Disclosure List.

Section 16 - Additional Information

MSDS Creation Date: 8/04/1998
Revision #7 Date: 10/03/2005

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.

Section 1 - Chemical Product and Company Identification
MSDS Name: Gram Decolorizer Solution 1:1
Catalog Numbers:
Synonyms:
Mixture.
Company Identification:

              Fisher Diagnostics
              Fisher Scientific Company, LLC
              8365 Valley Pike
              Middletown, VA 22645-0307

For information, call:
800-524-0294
Emergency Number:
800-524-0294
For CHEMTREC assistance, call:
800-424-9300
For International CHEMTREC assistance, call:
703-527-3887

Section 2 - Composition, Information on Ingredients

CAS# Chemical Name Percent EINECS/ELINCS
67-64-1 Acetone 50 200-662-2
64-17-5 Ethyl alcohol 42.75 200-578-6
7732-18-5 Deionized water 5.25 231-791-2
67-56-1 Methyl alcohol 2 200-659-6

Section 3 - Hazards Identification

EMERGENCY OVERVIEW

Appearance: clear, colorless liquid. Flash Point: 32 deg F.
Warning! Causes respiratory tract irritation. Causes eye irritation. Breathing vapors may cause drowsiness and dizziness. Flammable liquid and vapor. Prolonged or repeated contact may dry the skin and cause irritation.
Target Organs: Central nervous system, respiratory system, eyes, skin.


Potential Health Effects
Eye: Produces irritation, characterized by a burning sensation, redness, tearing, inflammation, and possible corneal injury.
Skin: May be absorbed through the skin. Repeated or prolonged exposure may cause drying and cracking of the skin.
Ingestion: May cause irritation of the digestive tract. May cause central nervous system depression, characterized by excitement, followed by headache, dizziness, drowsiness, and nausea. Advanced stages may cause collapse, unconsciousness, coma and possible death due to respiratory failure.
Inhalation: Inhalation of high concentrations may cause central nervous system effects characterized by nausea, headache, dizziness, unconsciousness and coma. Causes respiratory tract irritation. May cause motor incoordination and speech abnormalities.
Chronic: Prolonged or repeated skin contact may cause dermatitis. Chronic inhalation may cause effects similar to those of acute inhalation.

Section 4 - First Aid Measures


Eyes: Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid immediately.
Skin: Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Get medical aid if irritation develops or persists. Wash clothing before reuse.
Ingestion: Do not induce vomiting. If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid immediately.
Inhalation: Get medical aid immediately. Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid. Do NOT use mouth-to-mouth resuscitation. If breathing has ceased apply artificial respiration using oxygen and a suitable mechanical device such as a bag and a mask.
Notes to Physician: Treat symptomatically and supportively.

Section 5 - Fire Fighting Measures


General Information: Containers can build up pressure if exposed to heat and/or fire. As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Use water spray to keep fire-exposed containers cool. May be ignited by heat, sparks, and flame. Vapors are heavier than air and may travel to a source of ignition and flash back. Vapors can spread along the ground and collect in low or confined areas.
Extinguishing Media: For small fires, use dry chemical, carbon dioxide, water spray or alcohol-resistant foam. For large fires, use water spray, fog, or alcohol-resistant foam. Use water spray to cool fire-exposed containers. Water may be ineffective. Do NOT use straight streams of water. Cool containers with flooding quantities of water until well after fire is out.
Flash Point: 32e deg F ( 0.00 deg C)
Autoignition Temperature: Not applicable.
Explosion Limits, Lower:2.2 vol %
Upper: 13.0 vol %
NFPA Rating: (estimated) Health: 2; Flammability: 3; Instability: 0

Section 6 - Accidental Release Measures


General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Absorb spill with inert material (e.g. vermiculite, sand or earth), then place in suitable container. Avoid runoff into storm sewers and ditches which lead to waterways. Wear appropriate protective clothing to minimize contact with skin. Remove all sources of ignition. Provide ventilation. A vapor suppressing foam may be used to reduce vapors. Water spray may reduce vapor but may not prevent ignition in closed spaces. Clean up residual material by washing area with a 2-5% solution of soda ash.

Section 7 - Handling and Storage


Handling: Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Ground and bond containers when transferring material. Avoid contact with eyes, skin, and clothing. Empty containers retain product residue, (liquid and/or vapor), and can be dangerous. Keep container tightly closed. Avoid ingestion and inhalation. Do not pressurize, cut, weld, braze, solder, drill, grind, or expose empty containers to heat, sparks or open flames. Use only with adequate ventilation. Keep away from heat, sparks and flame.
Storage: Keep away from heat, sparks, and flame. Keep away from sources of ignition. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances. Flammables-area.

Section 8 - Exposure Controls, Personal Protection


Engineering Controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate general or local exhaust ventilation to keep airborne concentrations below the permissible exposure limits.
Exposure Limits
Chemical Name ACGIH NIOSH OSHA - Final PELs
Acetone 500 ppm TWA; 750 ppm STEL 250 ppm TWA; 590 mg/m3 TWA 2500 ppm IDLH 1000 ppm TWA; 2400 mg/m3 TWA
Ethyl alcohol 1000 ppm TWA 1000 ppm TWA; 1900 mg/m3 TWA 3300 ppm IDLH 1000 ppm TWA; 1900 mg/m3 TWA
Deionized water none listed none listed none listed
Methyl alcohol 200 ppm TWA; 250 ppm STEL; Skin - potential significant contribution to overall exposure by the cutaneous r oute 200 ppm TWA; 260 mg/m3 TWA 6000 ppm IDLH 200 ppm TWA; 260 mg/m3 TWA

OSHA Vacated PELs: Acetone: 750 ppm TWA; 1800 mg/m3 TWA Ethyl alcohol: 1000 ppm TWA; 1900 mg/m3 TWA Deionized water: No OSHA Vacated PELs are listed for this chemical. Methyl alcohol: 200 ppm TWA; 260 mg/m3 TWA
Personal Protective Equipment
Eyes: Wear chemical splash goggles.
Skin: Wear appropriate protective gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

Section 9 - Physical and Chemical Properties


Physical State: Liquid
Appearance: clear, colorless
Odor: Pungent
pH: Not available.
Vapor Pressure: Not available.
Vapor Density: Not available.
Evaporation Rate:Not available.
Viscosity: Not available.
Boiling Point: 133 deg F
Freezing/Melting Point:-116 deg C
Decomposition Temperature:Not available.
Solubility: Soluble.
Specific Gravity/Density:0.794 @ 25C
Molecular Formula:Not available.
Molecular Weight:Not available.

Section 10 - Stability and Reactivity


Chemical Stability: Stable at room temperature in closed containers under normal storage and handling conditions.
Conditions to Avoid: High temperatures, ignition sources, temperatures above 220C.
Incompatibilities with Other Materials: Strong oxidizing agents, strong acids.
Hazardous Decomposition Products: Carbon monoxide, irritating and toxic fumes and gases, carbon dioxide.
Hazardous Polymerization: Will not occur.

Section 11 - Toxicological Information


RTECS#:      
CAS# 67-64-1: AL3150000      
CAS# 64-17-5: KQ6300000      
CAS# 7732-18-5: ZC0110000      
CAS# 67-56-1: PC1400000
LD50/LC50:
CAS# 67-64-1:
     Dermal, guinea pig: LD50 = >9400 uL/kg;
     Draize test, rabbit, eye: 20 mg Severe;
     Draize test, rabbit, eye: 20 mg/24H Moderate;
     Draize test, rabbit, eye: 10 uL Mild;
     Draize test, rabbit, skin: 500 mg/24H Mild;
     Inhalation, mouse: LC50 = 44 gm/m3/4H;
     Inhalation, rat: LC50 = 50100 mg/m3/8H;
     Oral, mouse: LD50 = 3 gm/kg;
     Oral, rabbit: LD50 = 5340 mg/kg;
     Oral, rat: LD50 = 5800 mg/kg;
.

CAS# 64-17-5:
     Draize test, rabbit, eye: 500 mg Severe;
     Draize test, rabbit, eye: 500 mg/24H Mild;
     Draize test, rabbit, skin: 20 mg/24H Moderate;
     Inhalation, mouse: LC50 = 39 gm/m3/4H;
     Inhalation, rat: LC50 = 20000 ppm/10H;
     Oral, mouse: LD50 = 3450 mg/kg;
     Oral, rabbit: LD50 = 6300 mg/kg;
     Oral, rat: LD50 = 7060 mg/kg;
     Oral, rat: LD50 = 9000 mg/kg;
.

CAS# 7732-18-5:
     Oral, rat: LD50 = >90 mL/kg;
.

CAS# 67-56-1:
     Draize test, rabbit, eye: 40 mg Moderate;
     Draize test, rabbit, eye: 100 mg/24H Moderate;
     Draize test, rabbit, skin: 20 mg/24H Moderate;
     Inhalation, rabbit: LC50 = 81000 mg/m3/14H;
     Inhalation, rat: LC50 = 64000 ppm/4H;
     Oral, mouse: LD50 = 7300 mg/kg;
     Oral, rabbit: LD50 = 14200 mg/kg;
     Oral, rat: LD50 = 5600 mg/kg;
     Skin, rabbit: LD50 = 15800 mg/kg;
.

Carcinogenicity:
CAS# 67-64-1: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 64-17-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 7732-18-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 67-56-1: Not listed by ACGIH, IARC, NTP, or CA Prop 65.

Epidemiology: No information found
Teratogenicity: See actual entry in RTECS for complete information.
Reproductive Effects: TDLo(Oral, rat) = 273 gm/kg;Reproductive - Paternal Effects - spermatogenesis (incl. genetic material, sperm morphology, motility, and count).
Mutagenicity: Sex chromosome loss and nondisjunction(Yeast - Saccharomyces cerevisiae) = 47600 ppm; Cytogenetic analysis(Rodent - hamster Fibroblast)= 40 gm/L.
Neurotoxicity: See actual entry in RTECS for complete information.
Other Studies:

Section 12 - Ecological Information

No information available.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series:
CAS# 67-64-1: waste number U002 (Ignitable waste).
CAS# 67-56-1: waste number U154 (Ignitable waste).

Section 14 - Transport Information

US DOT Canada TDG
Shipping Name: FLAMMABLE LIQUIDS, N.O.S. No information available.
Hazard Class: 3
UN Number: UN1993
Packing Group: II

Section 15 - Regulatory Information

US FEDERAL

TSCA
     CAS# 67-64-1 is listed on the TSCA inventory.
     CAS# 64-17-5 is listed on the TSCA inventory.
     CAS# 7732-18-5 is listed on the TSCA inventory.
     CAS# 67-56-1 is listed on the TSCA inventory.
Health & Safety Reporting List
     None of the chemicals are on the Health & Safety Reporting List.
Chemical Test Rules
     CAS# 67-64-1: Test for Health Effects
Section 12b
     None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
     None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
     CAS# 67-64-1: 5000 lb final RQ; 2270 kg final RQ      CAS# 67-56-1: 5000 lb final RQ; 2270 kg final RQ
SARA Section 302 Extremely Hazardous Substances
     None of the chemicals in this product have a TPQ.
SARA Codes
     CAS # 67-64-1: immediate, fire.
     CAS # 64-17-5: immediate, delayed, fire.
     CAS # 67-56-1: immediate, fire.
Section 313
     This material contains Methyl alcohol (CAS# 67-56-1, 2%),which is subject to the reporting requirements of Section 313 of SARA Title III and 40 CFR Part 373.
Clean Air Act:
     CAS# 67-56-1 is listed as a hazardous air pollutant (HAP).
     This material does not contain any Class 1 Ozone depletors.
     This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
     None of the chemicals in this product are listed as Hazardous Substances under the CWA.
     None of the chemicals in this product are listed as Priority Pollutants under the CWA.
     None of the chemicals in this product are listed as Toxic Pollutants under the CWA.
OSHA:
     None of the chemicals in this product are considered highly hazardous by OSHA.
STATE
     CAS# 67-64-1 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 64-17-5 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 7732-18-5 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
     CAS# 67-56-1 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.

California Prop 65
WARNING: This product contains Ethyl alcohol, a chemical known to the state of California to cause developmental reproductive toxicity.
California No Significant Risk Level: None of the chemicals in this product are listed.

European/International Regulations

European Labeling in Accordance with EC Directives
Hazard Symbols:
     XI F
Risk Phrases:

     R 11 Highly flammable.
     R 36 Irritating to eyes.
     R 66 Repeated exposure may cause skin dryness or cracking.
     R 67 Vapours may cause drowsiness and dizziness.

Safety Phrases:

     S 16 Keep away from sources of ignition - No smoking.
     S 26 In case of contact with eyes, rinse immediately with plenty of
     water and seek medical advice.
     S 9 Keep container in a well-ventilated place.

WGK (Water Danger/Protection)

     CAS# 67-64-1: 0
     CAS# 64-17-5: 0
     CAS# 7732-18-5: No information available.
     CAS# 67-56-1: 1
Canada - DSL/NDSL
     CAS# 67-64-1 is listed on Canada's DSL List.
     CAS# 64-17-5 is listed on Canada's DSL List.
     CAS# 7732-18-5 is listed on Canada's DSL List.
     CAS# 67-56-1 is listed on Canada's DSL List.
Canada - WHMIS
     This product has a WHMIS classification of B2, D2B.
This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations.
Canadian Ingredient Disclosure List
     CAS# 67-64-1 is listed on the Canadian Ingredient Disclosure List.
     CAS# 64-17-5 is listed on the Canadian Ingredient Disclosure List.
     CAS# 67-56-1 is listed on the Canadian Ingredient Disclosure List.

Section 16 - Additional Information

MSDS Creation Date: 3/18/1999
Revision #7 Date: 10/05/2004

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.

Section 1 - Chemical Product and Company Identification
MSDS Name: Crystal Violet Solution
Catalog Numbers: 23255960, 23270180, 23291472, 66761
Synonyms:
None.
Company Identification:

              Fisher Diagnostics
              Fisher Scientific Company, LLC
              8365 Valley Pike
              Middletown, VA 22645-0307

For information, call:
800-524-0294
Emergency Number:
800-524-0294
For CHEMTREC assistance, call:
800-424-9300
For International CHEMTREC assistance, call:
703-527-3887

Section 2 - Composition, Information on Ingredients

CAS# Chemical Name Percent EINECS/ELINCS
7732-18-5 Water >85 231-791-2
64-17-5 Ethanol <12 200-578-6
67-56-1 Methanol <1 200-659-6
108-95-2 Phenol <1 203-632-7
548-62-9 Crystal Violet 0.4 208-953-6

Section 3 - Hazards Identification

EMERGENCY OVERVIEW

Appearance: purple liquid. Flash Point: 120 deg F.
Warning! Causes eye irritation. Flammable liquid and vapor. May cause skin and respiratory tract irritation. May cause central nervous system depression. May cause liver and kidney damage. May cause reproductive and fetal effects.
Target Organs: Kidneys, central nervous system, liver.


Potential Health Effects
Eye: Causes eye irritation. Produces irritation, characterized by a burning sensation, redness, tearing, inflammation, and possible corneal injury.
Skin: May cause skin irritation. May be absorbed through the skin.
Ingestion: May cause irritation of the digestive tract. May cause central nervous system depression, kidney damage, and liver damage. May cause systemic toxicity with acidosis. May cause liver and kidney damage.
Inhalation: Inhalation of high concentrations may cause central nervous system effects characterized by nausea, headache, dizziness, unconsciousness and coma. May cause respiratory tract irritation. Prolonged exposure may result in dizziness and general weakness.
Chronic: Chronic inhalation and ingestion may cause effects similar to those of acute inhalation and ingestion.

Section 4 - First Aid Measures


Eyes: In case of contact, immediately flush eyes with plenty of water for a t least 15 minutes. Get medical aid.
Skin: In case of contact, flush skin with plenty of water. Remove contaminated clothing and shoes. Get medical aid if irritation develops and persists. Wash clothing before reuse.
Ingestion: If swallowed, do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical aid.
Inhalation: If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid.
Notes to Physician: Treat symptomatically and supportively.

Section 5 - Fire Fighting Measures


General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. Use water spray to keep fire-exposed containers cool. Flammable liquid and vapor.
Extinguishing Media: Use alcohol foam, carbon dioxide, or water spray when fighting fires involving this material.
Flash Point: 120e deg F ( 48.89 deg C)
Autoignition Temperature: Not available.
Explosion Limits, Lower:Not available.
Upper: Not available.
NFPA Rating: (estimated) Health: 2; Flammability: 2; Instability: 0

Section 6 - Accidental Release Measures


General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Absorb spill with inert material (e.g. vermiculite, sand or earth), then place in suitable container. Remove all sources of ignition. Provide ventilation.

Section 7 - Handling and Storage


Handling: Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Avoid contact with eyes, skin, and clothing. Use only with adequate ventilation. Keep away from heat, sparks and flame. Avoid breathing vapor or mist.
Storage: Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances. Flammables-area.

Section 8 - Exposure Controls, Personal Protection


Engineering Controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate general or local exhaust ventilation to keep airborne concentrations below the permissible exposure limits.
Exposure Limits
Chemical Name ACGIH NIOSH OSHA - Final PELs
Water none listed none listed none listed
Ethanol 1000 ppm TWA 1000 ppm TWA; 1900 mg/m3 TWA 3300 ppm IDLH 1000 ppm TWA; 1900 mg/m3 TWA
Methanol 200 ppm TWA; 250 ppm STEL; Skin - potential significant contribution to overall exposure by the cutaneous r oute 200 ppm TWA; 260 mg/m3 TWA 6000 ppm IDLH 200 ppm TWA; 260 mg/m3 TWA
Phenol 5 ppm TWA; Skin - potential significant contribution to overall exposure by the cutaneous r oute 5 ppm TWA; 19 mg/m3 TWA 250 ppm IDLH 5 ppm TWA; 19 mg/m3 TWA
Crystal Violet none listed none listed none listed

OSHA Vacated PELs: Water: No OSHA Vacated PELs are listed for this chemical. Ethanol: 1000 ppm TWA; 1900 mg/m3 TWA Methanol: 200 ppm TWA; 260 mg/m3 TWA Phenol: 5 ppm TWA; 19 mg/m3 TWA Crystal Violet: No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear chemical splash goggles.
Skin: Wear appropriate protective gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

Section 9 - Physical and Chemical Properties


Physical State: Liquid
Appearance: purple
Odor: Alcoholic odor.
pH: Not available.
Vapor Pressure: 40 mm Hg @19 C
Vapor Density: 1.59 (Air=1)
Evaporation Rate:Not available.
Viscosity: Not available.
Boiling Point: 98 deg C
Freezing/Melting Point:-114 deg C
Decomposition Temperature:Not available.
Solubility: Complete in water.
Specific Gravity/Density:1 (Water=1)
Molecular Formula:Not applicable.
Molecular Weight:Not available.

Section 10 - Stability and Reactivity


Chemical Stability: Stable under normal temperatures and pressures.
Conditions to Avoid: Ignition sources.
Incompatibilities with Other Materials: Strong oxidizing agents.
Hazardous Decomposition Products: Carbon monoxide, carbon dioxide.
Hazardous Polymerization: Will not occur.

Section 11 - Toxicological Information


RTECS#:      
CAS# 7732-18-5: ZC0110000      
CAS# 64-17-5: KQ6300000      
CAS# 67-56-1: PC1400000      
CAS# 108-95-2: SJ3325000      
CAS# 548-62-9: BO9000000
LD50/LC50:
CAS# 7732-18-5:
     Oral, rat: LD50 = >90 mL/kg;
.

CAS# 64-17-5:
     Draize test, rabbit, eye: 500 mg Severe;
     Draize test, rabbit, eye: 500 mg/24H Mild;
     Draize test, rabbit, skin: 20 mg/24H Moderate;
     Inhalation, mouse: LC50 = 39 gm/m3/4H;
     Inhalation, rat: LC50 = 20000 ppm/10H;
     Oral, mouse: LD50 = 3450 mg/kg;
     Oral, rabbit: LD50 = 6300 mg/kg;
     Oral, rat: LD50 = 7060 mg/kg;
     Oral, rat: LD50 = 9000 mg/kg;
.

CAS# 67-56-1:
     Draize test, rabbit, eye: 40 mg Moderate;
     Draize test, rabbit, eye: 100 mg/24H Moderate;
     Draize test, rabbit, skin: 20 mg/24H Moderate;
     Inhalation, rabbit: LC50 = 81000 mg/m3/14H;
     Inhalation, rat: LC50 = 64000 ppm/4H;
     Oral, mouse: LD50 = 7300 mg/kg;
     Oral, rabbit: LD50 = 14200 mg/kg;
     Oral, rat: LD50 = 5600 mg/kg;
     Skin, rabbit: LD50 = 15800 mg/kg;
.

CAS# 108-95-2:
     Draize test, rabbit, eye: 5 mg Severe;
     Draize test, rabbit, skin: 500 mg/24H Severe;
     Draize test, rabbit, skin: 100 mg Mild;
     Inhalation, mouse: LC50 = 177 mg/m3;
     Inhalation, mouse: LC50 = 177 mg/m3/4H;
     Inhalation, rat: LC50 = 316 mg/m3;
     Inhalation, rat: LC50 = 316 mg/m3/4H;
     Oral, mouse: LD50 = 270 mg/kg;
     Oral, rat: LD50 = 317 mg/kg;
     Oral, rat: LD50 = 512 mg/kg;
     Skin, rabbit: LD50 = 630 mg/kg;
     Skin, rat: LD50 = 669 mg/kg;
     Skin, rat: LD50 = 1500 mg/kg;
.

CAS# 548-62-9:
     Oral, mouse: LD50 = 96 mg/kg;
     Oral, rabbit: LD50 = 150 mg/kg;
     Oral, rat: LD50 = 420 mg/kg;
.

Carcinogenicity:
CAS# 7732-18-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 64-17-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 67-56-1: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 108-95-2: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 548-62-9: Not listed by ACGIH, IARC, NTP, or CA Prop 65.

Epidemiology: No information found
Teratogenicity: Repeated ingestion of ethanol by pregnant mothers has been shown to adversely affect the central nervous system of the fetus, producing a collection of effects which together constitute the fetal alcohol syndrome.
Reproductive Effects: No information found
Mutagenicity: No information found
Neurotoxicity: No information found
Other Studies:

Section 12 - Ecological Information

No information available.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series:
CAS# 67-56-1: waste number U154 (Ignitable waste).
CAS# 108-95-2: waste number U188.

Section 14 - Transport Information

US DOT Canada TDG
Shipping Name: NOT REGULATED FOR DOMESTIC TRANSPORT ETHANOL SOLUTIONS
Hazard Class: 3
UN Number: UN1170
Packing Group: III

Section 15 - Regulatory Information

US FEDERAL

TSCA
     CAS# 7732-18-5 is listed on the TSCA inventory.
     CAS# 64-17-5 is listed on the TSCA inventory.
     CAS# 67-56-1 is listed on the TSCA inventory.
     CAS# 108-95-2 is listed on the TSCA inventory.
     CAS# 548-62-9 is listed on the TSCA inventory.
Health & Safety Reporting List
     CAS# 108-95-2: Effective 6/1/87, Sunset 6/1/97
Chemical Test Rules
     None of the chemicals in this product are under a Chemical Test Rule.
Section 12b
     None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
     None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
     CAS# 67-56-1: 5000 lb final RQ; 2270 kg final RQ      CAS# 108-95-2: 1000 lb final RQ; 454 kg final RQ
SARA Section 302 Extremely Hazardous Substances
     CAS# 108-95-2: 500 lb TPQ (lower threshold); 10000 lb TPQ (upper thres      hold)
SARA Codes
     CAS # 64-17-5: immediate, delayed, fire.
     CAS # 67-56-1: immediate, fire.
     CAS # 108-95-2: immediate, delayed, fire.
     CAS # 548-62-9: immediate.
Section 313
     Methanol is not at a high enough concentration to be reportable under Section 313.
     Phenol is not at a high enough concentration to be reportable under Section 313.      No chemicals are reportable under Section 313.
Clean Air Act:
     CAS# 67-56-1 is listed as a hazardous air pollutant (HAP).
     CAS# 108-95-2 is listed as a hazardous air pollutant (HAP).

     This material does not contain any Class 1 Ozone depletors.
     This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
CAS# 108-95-2 is listed as a Hazardous Substance under the CWA.      CAS# 108-95-2 is listed as a Priority Pollutant under the Clean Water      Act. CAS# 108-95-2 is listed as a Toxic Pollutant under the Clean Water Act.
OSHA:
     None of the chemicals in this product are considered highly hazardous by OSHA.
STATE
     CAS# 7732-18-5 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
     CAS# 64-17-5 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 67-56-1 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 108-95-2 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 548-62-9 is not present on state lists from CA, PA, MN, MA, FL, or NJ.

California Prop 65
WARNING: This product contains Ethanol, a chemical known to the state of California to cause developmental reproductive toxicity.
California No Significant Risk Level: None of the chemicals in this product are listed.

European/International Regulations

European Labeling in Accordance with EC Directives
Hazard Symbols:
      Not available.
Risk Phrases:


Safety Phrases:


WGK (Water Danger/Protection)

     CAS# 7732-18-5: No information available.
     CAS# 64-17-5: 0
     CAS# 67-56-1: 1
     CAS# 108-95-2: 2
     CAS# 548-62-9: 2
Canada - DSL/NDSL
     CAS# 7732-18-5 is listed on Canada's DSL List.
     CAS# 64-17-5 is listed on Canada's DSL List.
     CAS# 67-56-1 is listed on Canada's DSL List.
     CAS# 108-95-2 is listed on Canada's DSL List.
     CAS# 548-62-9 is listed on Canada's DSL List.
Canada - WHMIS
     This product has a WHMIS classification of B3.
This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations.
Canadian Ingredient Disclosure List
     CAS# 64-17-5 is listed on the Canadian Ingredient Disclosure List.
     CAS# 67-56-1 is listed on the Canadian Ingredient Disclosure List.
     CAS# 108-95-2 is listed on the Canadian Ingredient Disclosure List.
     CAS# 548-62-9 is listed on the Canadian Ingredient Disclosure List.

Section 16 - Additional Information

MSDS Creation Date: 7/28/1998
Revision #13 Date: 2/18/2004

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.

Section 1 - Chemical Product and Company Identification
MSDS Name: Iodine
Catalog Numbers: 2300584, 2300585, 23281407B, 66770
Synonyms:
Mixture.
Company Identification:

              Fisher Diagnostics
              Fisher Scientific Company, LLC
              8365 Valley Pike
              Middletown, VA 22645-0307

For information, call:
800-524-0294
Emergency Number:
800-524-0294
For CHEMTREC assistance, call:
800-424-9300
For International CHEMTREC assistance, call:
703-527-3887

Section 2 - Composition, Information on Ingredients

CAS# Chemical Name Percent EINECS/ELINCS
25655-41-8 Povidone-Iodine <13.0% unlisted
7681-11-0 Potassium Iodide 1.9 231-659-4
7732-18-5 Deionized Water Balance 231-791-2

Section 3 - Hazards Identification

EMERGENCY OVERVIEW

Appearance: dark red-brown liquid.
Warning! May cause allergic skin reaction. Causes eye and skin irritation. Causes digestive and respiratory tract irritation. Eye contact may result in permanent eye damage. May cause fetal effects based upon animal studies.
Target Organs: Thyroid.


Potential Health Effects
Eye: Causes eye irritation and possible injury.
Skin: Causes skin irritation. May cause skin sensitization, an allergic reaction, which becomes evident upon re-exposure to this material.
Ingestion: Causes gastrointestinal irritation with nausea, vomiting and diarrhea. Chronic ingestion of iodides during pregnancy has resulted in fetal death, severe goiter, and cretinoid appearance of the newborn.
Inhalation: Causes respiratory tract irritation.
Chronic: Chronic exposure can lead to iodism characterized by salivation, nasal discharge, sneezing, conjunctivitis, fever, laryngitis, bronchitis, stomatitis, and skin rashes. May interfere with iodine uptake of the thyroid gland and enlarge it. Chronic ingestion of iodides during pregnancy has resulted in fetal death, severe goiter, and cretinoid appearance of the newborn.. Prolonged exposure to iodides may produce iodism in sensitive individuals. Symptoms could include skin rash, running nose and headache.

Section 4 - First Aid Measures


Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid.
Skin: Get medical aid. Wash clothing before reuse. Flush skin with plenty of soap and water.
Ingestion: Do not induce vomiting. If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid immediately.
Inhalation: Remove from exposure and move to fresh air immediately. If breathing is difficult, give oxygen. Get medical aid. Do NOT use mouth-to-mouth resuscitation. If breathing has ceased apply artificial respiration using oxygen and a suitable mechanical device such as a bag and a mask.
Notes to Physician: Treat symptomatically and supportively.

Section 5 - Fire Fighting Measures


General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Non-combustible, substance itself does not burn but may decompose upon heating to produce irritating, corrosive and/or toxic fumes.
Extinguishing Media: Substance is noncombustible; use agent most appropriate to extinguish surrounding fire.
Flash Point: Not applicable.
Autoignition Temperature: Not applicable.
Explosion Limits, Lower:Not available.
Upper: Not available.
NFPA Rating: (estimated) Health: 2; Flammability: 0; Instability: 0

Section 6 - Accidental Release Measures


General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Absorb spill with inert material (e.g. vermiculite, sand or earth), then place in suitable container. Avoid runoff into storm sewers and ditches which lead to waterways. Clean up spills immediately, observing precautions in the Protective Equipment section. Provide ventilation.

Section 7 - Handling and Storage


Handling: Wash thoroughly after handling. Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Avoid ingestion and inhalation.
Storage: Store in a cool, dry, well-ventilated area away from incompatible substances.

Section 8 - Exposure Controls, Personal Protection


Engineering Controls: Good general ventilation should be sufficient to control airborne levels. Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower.
Exposure Limits
Chemical Name ACGIH NIOSH OSHA - Final PELs
Povidone-Iodine none listed none listed none listed
Potassium Iodide none listed none listed none listed
Deionized Water none listed none listed none listed

OSHA Vacated PELs: Povidone-Iodine: No OSHA Vacated PELs are listed for this chemical. Potassium Iodide: No OSHA Vacated PELs are listed for this chemical. Deionized Water: No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Wear appropriate protective gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

Section 9 - Physical and Chemical Properties


Physical State: Liquid
Appearance: dark red-brown
Odor: Iodine
pH: Not available.
Vapor Pressure: Not available.
Vapor Density: >1.00
Evaporation Rate:Not available.
Viscosity: Not available.
Boiling Point: 100 deg C
Freezing/Melting Point:> 0 deg C
Decomposition Temperature:Not available.
Solubility: Soluble in water.
Specific Gravity/Density:~1
Molecular Formula:Solution
Molecular Weight:Not available.

Section 10 - Stability and Reactivity


Chemical Stability: Stable under normal temperatures and pressures.
Conditions to Avoid: Incompatible materials, excess heat.
Incompatibilities with Other Materials: Incompatabilities with potassium iodide include: salts of alkaloids, chloral hydrate, calomel(mercurous chloride) potassium chlorate, metallic salts, tartaric and other acids, bromine trifluoride, and fluorine perchlorate, oxidizing agents. Incompatibilities with PVP include oxidizing agents.
Hazardous Decomposition Products: Irritating and toxic fumes and gases, oxides of potassium, iodine.
Hazardous Polymerization: Will not occur.

Section 11 - Toxicological Information


RTECS#:      
CAS# 25655-41-8: TR1579600      
CAS# 7681-11-0: TT2975000      
CAS# 7732-18-5: ZC0110000
LD50/LC50:
CAS# 25655-41-8:
     Draize test, rabbit, skin: 500 mg Mild;
     Oral, mouse: LD50 = 8100 mg/kg;
     Oral, rat: LD50 = >8 gm/kg;
.

CAS# 7681-11-0:
.

CAS# 7732-18-5:
     Oral, rat: LD50 = >90 mL/kg;
.

Carcinogenicity:
CAS# 25655-41-8: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 7681-11-0: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 7732-18-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.

Epidemiology: Experimental reproductive effects have been reported.
Teratogenicity: Iodine salts can produce deformity, illness, and death of a fetus. PVP(iodine complex) has been shown to present a possible risk of harm to the unborn child.
Reproductive Effects: PVP(iodine complex) has been shown to produce reproductive effects when there is overexposure.
Mutagenicity: No information found
Neurotoxicity: No information found
Other Studies:

Section 12 - Ecological Information

No information available.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series: None listed.

Section 14 - Transport Information

US DOT Canada TDG
Shipping Name: Not regulated as a hazardous material No information available.
Hazard Class:
UN Number:
Packing Group:

Section 15 - Regulatory Information

US FEDERAL

TSCA
     CAS# 25655-41-8 is listed on the TSCA inventory.
     CAS# 7681-11-0 is listed on the TSCA inventory.
     CAS# 7732-18-5 is listed on the TSCA inventory.
Health & Safety Reporting List
     None of the chemicals are on the Health & Safety Reporting List.
Chemical Test Rules
     None of the chemicals in this product are under a Chemical Test Rule.
Section 12b
     None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
     None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
     None of the chemicals in this material have an RQ.
SARA Section 302 Extremely Hazardous Substances
     None of the chemicals in this product have a TPQ.
SARA Codes
     CAS # 7681-11-0: immediate, delayed.
Section 313      No chemicals are reportable under Section 313.
Clean Air Act:
     This material does not contain any hazardous air pollutants.
     This material does not contain any Class 1 Ozone depletors.
     This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
     None of the chemicals in this product are listed as Hazardous Substances under the CWA.
     None of the chemicals in this product are listed as Priority Pollutants under the CWA.
     None of the chemicals in this product are listed as Toxic Pollutants under the CWA.
OSHA:
     None of the chemicals in this product are considered highly hazardous by OSHA.
STATE
     CAS# 25655-41-8 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
     CAS# 7681-11-0 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
     CAS# 7732-18-5 is not present on state lists from CA, PA, MN, MA, FL, or NJ.

California Prop 65

California No Significant Risk Level: None of the chemicals in this product are listed.

European/International Regulations

European Labeling in Accordance with EC Directives
Hazard Symbols:
      Not available.
Risk Phrases:


Safety Phrases:


WGK (Water Danger/Protection)

     CAS# 25655-41-8: 2
     CAS# 7681-11-0: 1
     CAS# 7732-18-5: No information available.
Canada - DSL/NDSL
     CAS# 25655-41-8 is listed on Canada's DSL List.
     CAS# 7681-11-0 is listed on Canada's DSL List.
     CAS# 7732-18-5 is listed on Canada's DSL List.
Canada - WHMIS
     This product has a WHMIS classification of D2A.
This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations.
Canadian Ingredient Disclosure List
     CAS# 7681-11-0 is listed on the Canadian Ingredient Disclosure List.

Section 16 - Additional Information

MSDS Creation Date: 3/25/1998
Revision #7 Date: 10/03/2005

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.

Section 1 - Chemical Product and Company Identification
MSDS Name: Decolorizing Solution. Component of Gram Stain Kits with stabilized and nonstabilized iodine.
Catalog Numbers: 23255962, 23270182, 23281407C, 23291474, 255962, 270182, 281407C, 291474, 66759E, 66760E, 66764, 66770D, 66780E, 66784
Synonyms:
None.
Company Identification:

              Fisher Diagnostics
              Fisher Scientific Company, LLC
              8365 Valley Pike
              Middletown, VA 22645-0307

For information, call:
800-524-0294
Emergency Number:
800-524-0294
For CHEMTREC assistance, call:
800-424-9300
For International CHEMTREC assistance, call:
703-527-3887

Section 2 - Composition, Information on Ingredients

CAS# Chemical Name Percent EINECS/ELINCS
67-63-0 Isopropyl alcohol 80 200-661-7
67-64-1 Acetone 20 200-662-2

Section 3 - Hazards Identification

EMERGENCY OVERVIEW

Appearance: clear, colorless liquid. Flash Point: 20 deg F.
Danger! Extremely flammable liquid and vapor. Vapor may cause flash fire. Breathing vapors may cause drowsiness and dizziness. Causes eye and respiratory tract irritation. Repeated exposure may cause skin dryness or cracking. Aspiration hazard if swallowed. Can enter lungs and cause damage. May form explosive peroxides.
Target Organs: Central nervous system, eyes, skin.


Potential Health Effects
Eye: Produces irritation, characterized by a burning sensation, redness, tearing, inflammation, and possible corneal injury.
Skin: Prolonged and/or repeated contact may cause defatting of the skin and dermatitis. May cause irritation with pain and stinging, especially if the skin is abraded.
Ingestion: May cause gastrointestinal irritation with nausea, vomiting and diarrhea. May cause central nervous system depression, characterized by excitement, followed by headache, dizziness, drowsiness, and nausea. Advanced stages may cause collapse, unconsciousness, coma and possible death due to respiratory failure. Aspiration of material into the lungs may cause chemical pneumonitis, which may be fatal. Possible aspiration hazard.
Inhalation: Inhalation of high concentrations may cause central nervous system effects characterized by nausea, headache, dizziness, unconsciousness and coma. Inhalation of vapor may cause respiratory tract irritation. May cause narcotic effects in high concentration.
Chronic: Prolonged or repeated skin contact may cause defatting and dermatitis.

Section 4 - First Aid Measures


Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid imme diately.
Skin: Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Get medical aid if irritation develops or persists. Wash clothing before reuse.
Ingestion: Potential for aspiration if swallowed. Get medical aid immediately. Do not induce vomiting unless directed to do so by medical

ALL Chemical Analysis PAGES IN THIS GROUP
NAMECAS
1-Cyclohexyl-3-(2-Morpholinoethyl)Carbodiimide Metho-P-Toluenesulfonate, 95% 2491-17-0
Congo Red (Amyloid Kit) 7732-18-5
LV-PVA 7732-18-5
3-Chloroanisole, 99% 2845-89-83-Chloroanisole<-td>
Surfactant 7732-18-5
2-Acetamidoacrylic acid, 99+ % 5429-56-1
Bianthrone 434-85-5
Alkaline Solution (Amyloid Kit) 7732-18-5
Schiff Reagent (Pas Kit) 7440-44-0
Red Counterstain (Iron , Retic. Kits) 7732-18-5
DAB Peroxide 7732-18-5
1-(4-Chlorophenyl)-1-cyclopentanecarbonyl chloride, 97% 71501-44-5
Oxidizer 7732-18-5
Acetic acid-d, 98 atom % d 758-12-3
n-Phenyl-1-naphthylamine, 98% 90-30-2
Eosin Y 7732-18-5
Absolute Alcohol 7732-18-5
Alcohol Blends 7732-18-5
Decolorizing Solution. Component of Gram Stain Kits with stabilized and nonstabilized iodine. 67-64-1
Acid Fast, Fluorescent Decolorizer Component 7647-01-0
5-Methyl-2-Nitrobenzyl Chloride 66424-91-7
TB Quick Stain-2 step Methylene Blue 7732-18-5
Automated IHC Peroxide Block 7732-18-5
Automated IHC Buffer #3 7732-18-5
Eosin Y, Innovation Instruments (BSI) 17372-87-1
Resin in Xylene 68240-09-5
Papanicolaou Stain OG-6 7732-18-5
50 % Reagent Alcohol, III 7732-18-5
Stabilized Iodine 7732-18-5
2-Hydroxy-4-Methoxybenzophenone, 98% 131-57-7
IHC Biotek, Automated IHC Universal Secondary Antibody 7732-18-5
Automated IHC Buffer #1 7732-18-5
Citrate Buffer, Auxiliary Reagents 7732-18-5
Myristyltrimethylammonium bromide, 99% (ti tr.) 1119-97-7
Eosin-Phloxine, III 7732-18-5



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